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Method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as explant

A technology of explants and embryonic axes, applied in the biological field, can solve problems such as poor repeatability, and achieve the effects of high rooting rate, wide application prospect and high repeatability

Inactive Publication Date: 2016-06-01
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the tissue culture of tung tree, mainly using the detached leaves and hypocotyls of tung tree as explants to obtain plant regeneration through the indirect organogenesis pathway and the petioles as explants to obtain plant regeneration through the direct organogenesis pathway. Plant regeneration, the research process has the problem of poor repeatability
At present, there is no report about the plant regeneration through the direct organogenesis pathway using the hypocotyl of tung aseptic seedlings as explants

Method used

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  • Method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as explant
  • Method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as explant
  • Method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as explant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Select seeds with uniform size and full grain, peel off the seed shell and rinse with running water for 15 minutes, during which time add 2ml of detergent. Wash the ultra-clean table with sterile water for 3 times, soak it in 75% alcohol for 60s, and then wash it with 0.1% HgCl 2 Process for 3 minutes, during which the bottle was shaken continuously. Then rinse with sterile water 4 to 5 times to fully wash away the residual disinfectant. After disinfection, soak the seeds in sterile water for 2 hours. Peel off the endosperm, and connect the stripped mature embryo of tung tree to the seed germination medium;

[0036] 2. Light culture 20~25d obtains the seedling of tung tree seedling, selects the hypocotyl of aseptic seedling and cuts into the segment of 0.3cm, inoculates into induction medium, is 25 ℃ in temperature, light intensity is 2000~2500lx, and light time is Under the condition of 14~16h / d, adventitious buds were induced ( figure 1 ); wherein the adventiti...

Embodiment 2

[0042] 1. Select seeds with uniform size and full grain, peel off the seed shell and rinse with running water for 20 minutes, adding 3ml of detergent during the process. Wash the ultra-clean bench with sterile water for 3 times, soak it in 75% alcohol for 90s, and then wash it with 0.1% HgCl 2 Process for 4 minutes, during which the bottle is kept shaking. Then rinse with sterile water 4 to 5 times to fully wash away the residual disinfectant. After disinfection, soak the seeds in sterile water for 4 hours. Peel off the endosperm, and connect the stripped mature embryo of tung tree to the seed germination medium;

[0043] 2. Light culture 20~25d obtains the seedling of tung tree seedling, selects hypocotyl of aseptic seedling and cuts into the segment of 0.5cm, inoculates into induction medium, is 25 ℃ in temperature, light intensity is 2000~2500lx, and light time is Adventitious buds were induced under the conditions of 14-16h / d, wherein the induction medium of adventitiou...

Embodiment 3

[0049] 1. Select seeds with uniform size and full grain, peel off the seed shell and rinse with running water for 30 minutes, adding 4ml of detergent during the period. Then wash the ultra-clean bench with sterile water for 3 times, soak it in 75% alcohol for 120s, and then wash it with 0.1% HgCl 2 Process for 6 minutes, during which the bottle was shaken continuously. Then rinse with sterile water 4 to 5 times to fully wash away the residual disinfectant. After disinfection, soak the seeds in sterile water for 6 hours. The endosperm is stripped off, and the stripped tung tree mature embryo is placed in a germination medium to germinate;

[0050] 2. Light culture 20~25d obtains the seedling of tung tree seedling, selects the hypocotyl of aseptic seedling and cuts into the segment of 0.6cm, inoculates into induction medium, is 25 ℃ in temperature, light intensity is 2000~2500lx, and light time is Adventitious bud induction is carried out under the condition of 14~16h / d; wher...

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Abstract

The invention discloses a method for directly inducing plant regeneration by using Vernicia fordii hypocotyl as an explant, comprising the following steps: using hypocotyl of a Vernicia fordii seed aseptic seedling as an explant, directly inducing an adventitious bud, proliferating and elongation culturing the adventitious bud, and performing rooting culturing, domestication and transplanting to obtain a robust regenerated plant of Vernicia fordii. Compared with the prior art, the method has the advantages such as regeneration process simplicity, hormone addition singleness, operation convenience and relatively short regeneration cycle. A new technical means to rapidly propagate Vernicia fordii is provided, and also certain technical support is provided for genetically improving Vernicia fordii by genetic engineering means in future.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for directly inducing plant regeneration by using tung tree hypocotyls as explants. Background technique [0002] Tung tree (Vernicia fordii Hemsley) is a deciduous tree belonging to the family Euphorbiaceae (Euphorbiaceae) and is a unique economic tree species in my country. Tung tung tree is an excellent bio-energy tree species. The oil content of its seeds is as high as 50% to 70%, of which about 94% are unsaturated fatty acids. It is a high-quality dry oil that can replace petrochemical diesel oil and has broad application prospects. The tung oil produced in my country is of high quality and enjoys a high reputation in the world. Its export output and quality both rank first in the world. With the rapid development of biomass energy forestry, the cultivation prospects of tung tree are very broad, and the market demand is large, but the lack of efficient seedling breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 吴丽芳穆燕侯金艳陈晶陆维丽毛颖基汤才国陈明
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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