Preparation method of renal biopsy electron microscopy sample

Abstract

The invention discloses a preparation method of a renal biopsy electron microscopy sample. The method comprises the following steps: transferring fixed renal biopsy tissues to a slide glass, placing the slide glass under a microscope with a light source, and cutting the tissues by using a double side blade to form a renal tissue segment with the length of 1-3mm and with glomerulus; cleaning the cut renal tissue, dehydrating the cleaned renal tissue, and immersing the dehydrated renal tissue; embedding the renal tissue; carrying out block trimming and positioning on the above obtained embedded and clustered block sample; accurately trimming and positioning the sample according to an electron microscopy observation result; and cutting the sample through using an ultrathin slicer to obtain an ultrathin slice. The preparation method of the renal biopsy electron microscopy sample allows adverse misbipsy samples to be found in the early stage and to be timely corrected, so repeated sample production is avoided; and the method also has the advantages of fast block trimming, short positioning time, accurate positioning, effective shortening of the sample preparation time, and substantial improvement of the diagnosable rate of renal biopsy samples and the diagnosis precision.

Description

technical field [0001] The invention relates to a method for preparing a kidney biopsy electron microscope sample. Background technique [0002] Renal biopsy is percutaneous renal biopsy, which is not only an important branch of pathology, but also an indispensable part of nephrology. Renal biopsy is very important for understanding the pathological changes, pathological types, and progression of kidney diseases and continuously optimizing individualized treatment plans. , Judging the prognosis is of great significance. In the 1950s, the application of electron microscopy in renal biopsy, especially transmission electron microscopy, allowed the observation of pathological changes in the kidney to the subcellular level. At present, renal biopsy electron microscopy has become one of the routine examination methods for the research and diagnosis of renal diseases, especially playing an irreplaceable role in the classification of primary glomerular diseases. For example, the t...

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Problems solved by technology

At present, renal biopsy electron microscopy has become one of the routine examination methods for the research and diagnosis of renal diseases, especially playing an irreplaceable role in the classification of primary glomerular diseases. For example, the tissue is skin or fat, or medulla without balls, etc.), as well as subsequent blind sample preparation of puncture specimens or routine longitudinal embedding of renal tissue segments, etc., resulting in errors in specimens only found during block trimming and semi-thin positioning observation Or it is difficult to locate the glomerulus, and the glomerulus structure is often not found under the electron microscope, so the diagnosis cannot be made, which wastes manpower and material resources, and delays the diagnosis time