Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma

A technology for auxiliary diagnosis of hepatocellular carcinoma, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problem of low sensitivity of liver cancer, achieve the effect of improving sensitivity, good prediction effect, and increasing the proportion of early diagnosis

Active Publication Date: 2016-05-25
CAPITALBIO CORP +1
View PDF4 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tumor marker clinically used to diagnose liver cancer is mainly alpha-fetoprotein (AFP), but about 40% of liver cancers do not secrete AFP, making the sensitivity of liver cancer detection generally low (20%-65%)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma
  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma
  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、8

[0048] Example 1, 8 miRNAs can be used to diagnose hepatocellular carcinoma

[0049] 1. Sample selection

[0050] The inventor of the present application collected plasma samples that meet the standards according to the standard operating procedure (SOP), systematically collected complete demographic data, clinical data, etc., and through sorting out the sample data, the inventor selected the plasma samples of 877 people as TLDA (TaqmanLowDensityArray, TLDA) chip detection and subsequent series of qRT-PCR verification experimental samples, these 877 people include 272 cases of hepatocellular carcinoma patients in the hepatocellular carcinoma group, 275 healthy people in the healthy control group, and benign liver disease group. 155 patients with benign liver diseases and 175 patients with benign and malignant other tissues and organs (including 30 cases of lung cancer, 30 cases of gastric cancer, 30 cases of colorectal cancer, 21 cases of esophageal cancer, 29 cases of benign ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a system for detecting the expression quantity of eight miRNAs in preparation of a product for diagnosing or assisting in diagnosing hepatocellular carcinoma. The hepatocellular carcinoma can be well diagnosed with a model built by the content of the eight RNAs including has-miR-20a, hsa-miR-21, hsa-miR-26a, hsa-miR-122, hsa-miR-192, hsa-miR-146a, hsa-miR-223 and hsa-miR-483-5 in blood plasma, the sensitivity can reach 69.6%, the specificity can reach 92.8%, the accuracy rate can reach 83.3%, and the sensitivity obtained when the eight miRNAs and AFP are utilized to perform joint diagnosis on the hepatocellular carcinoma can reach 85.7%. Therefore, the miRNAs in the blood plasma can diagnose the hepatocellular carcinoma by serving as a tumor diagnosis marker and also can diagnose the hepatocellular carcinoma together with the AFP.

Description

technical field [0001] The invention relates to the application of a system for detecting the expression levels of eight miRNAs in the preparation of products for diagnosis or auxiliary diagnosis of hepatocellular carcinoma in the field of biotechnology. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People usually refer to primary liver cancer as liver cancer. Primary liver cancer can be divided into hepatocellular carcinoma (hepatocellular carcinoma, HCC), intrahepatic cholangiocarcinoma and mixed liver cancer according to pathological classification, and HCC liver cancer accounts for about 90% of primary liver cancer. Primary liver cancer is one of the most common malignant tumors in clinical practice. According to the latest statistics, there are about 600,000 new liver cancer patients in the world every year, ranking fifth among malignant tumors. At present, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 郭弘妍杨春花董家鸿张爱群孙义民王亚辉邢婉丽程京
Owner CAPITALBIO CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com