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Preparation method of sialyl enteromorpha oligosaccharide promoting intestinal probiotics proliferation

A technology of intestinal probiotics and oligosaccharide prolifera, which is applied in the direction of chemicals for biological control, medical preparations containing active ingredients, and pharmaceutical formulations, to achieve the effect of improving commodity value, economic added value, and improving benefits

Active Publication Date: 2016-05-25
SHAANXI NIANQINGBAO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant patents and reports on the research on sialylated Enteromorpha oligosaccharides and the promotion of intestinal probiotics. The sialylated Enteromorpha oligosaccharides prepared by the present invention can significantly promote the proliferation of intestinal probiotics

Method used

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  • Preparation method of sialyl enteromorpha oligosaccharide promoting intestinal probiotics proliferation
  • Preparation method of sialyl enteromorpha oligosaccharide promoting intestinal probiotics proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) After drying, enteromorpha was ultrafinely crushed and passed through an 80-mesh sieve to obtain a dry powder. Add 60% ethanol to the dry powder of Enteromorpha and reflux for extraction for 1 hour. The ratio of dry powder weight to ethanol volume was 1:20. Recycle and remove ethanol after the reflux Recycle, dry, and pulverize to obtain dry defatted Enteromorpha powder;

[0022] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha to distilled water volume ratio is 1:20, 450W microwave-assisted extraction at an extraction temperature of 60°C for 2 minutes, and filter after the extraction is completed. The filter residue was extracted twice by repeating the above steps, the obtained filtrate was mixed and then concentrated, filtered with suction, the filtrate was concentrated under reduced pressure to 3% of the original volume, vacuum freeze-dried and crushed;

[0023] (3) Add distilled water to th...

Embodiment 2

[0032] (1) After drying, enteromorpha is ultrafinely crushed and passed through a 100-mesh sieve to obtain a dry powder. Add 80% ethanol to the dry powder of Enteromorpha and reflux for extraction for 2 hours. The ratio of dry powder weight to ethanol volume is 1:30. Recycle and remove ethanol after reflux Recycle, dry, and pulverize to obtain dry defatted Enteromorpha powder;

[0033] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha mass to distilled water volume is 1:25, 600W microwave-assisted extraction at an extraction temperature of 70°C for 3 minutes, and filter after extraction. The filter residue was extracted three times by repeating the above steps, the obtained filtrate was mixed and then concentrated, filtered with suction, the filtrate was concentrated under reduced pressure to 8% of the original volume, and then pulverized after vacuum freeze-drying;

[0034] (3) Add distilled water to the ...

Embodiment 3

[0043] (1) After drying, enteromorpha was ultrafinely pulverized and sieved through a 90-mesh sieve to obtain a dry powder. Add 70% ethanol to the dry powder of Enteromorpha reflux and extract for 1.5 hours. The ratio of dry powder weight to ethanol volume was 1:25, and recovered and removed after reflux Ethanol is recycled, dried, and pulverized to obtain dry and defatted Enteromorpha powder;

[0044] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the ratio of the mass of Enteromorpha to the volume of distilled water is 1:23, 500W microwave-assisted extraction at an extraction temperature of 65°C for 3 minutes, and filter after the extraction is completed. The filter residue was extracted three times by repeating the above steps, the obtained filtrate was mixed and then concentrated, filtered with suction, the filtrate was concentrated under reduced pressure to 5% of the original volume, and then pulverized after vacuum freeze-drying...

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Abstract

The invention discloses a preparation method of sialyl enteromorpha oligosaccharide promoting intestinal probiotics proliferation. Enteromorpha is dried and subjected to superfine grinding, and by means of an ethanol reflux extraction technology, the dried and degreased enteromorpha powder is obtained; the enteromorpha oligosaccharide is obtained through microwave-assisted extraction, deproteinization and polysaccharide and pigment removing; sialyltransferase is added into a reaction system containing enteromorpha oligosaccharide, a glycosylation reaction is conducted, and the sialyl enteromorpha oligosaccharide is prepared. The oligosaccharide can significantly increase the number of intestinal probiotics and can be used for preparing drugs or health-care products which improve intestinal flora.

Description

technical field [0001] The invention belongs to the technical field of health products, and in particular relates to a sialylated enteromorpha oligosaccharide for promoting the proliferation of intestinal probiotics and a preparation method thereof. Background technique [0002] There are a huge number of different types of bacteria in the human intestine, and the stability of the intestinal flora is essential for maintaining the health of the human body. Probiotics exert a beneficial effect on the host by improving the balance of intestinal microorganisms, including inhibiting the growth of pathogenic bacteria, multiplying and improving the structure of intestinal flora, enhancing the immune function of the body, lowering cholesterol and improving blood lipids, etc. Most of the probiotics come from the genus Bifidobacterium and Lactobacillus, and the reduction of the number of probiotics can cause the imbalance of the whole intestinal flora and intestinal microecology, thus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26A23L33/125A61K31/7032A01P1/00
Inventor 赵超吴一晶刘斌李秋哲杨成凤陈玉青
Owner SHAANXI NIANQINGBAO PHARMA CO LTD
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