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A kind of microalgae cultivation eco-regulator and using method thereof

An ecological regulator and microalgae culture technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, large labor and material resources, low efficiency, etc., and achieve low cost and high density. , the effect of long-term use

Active Publication Date: 2019-06-28
荣成恒顺海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this culture method is inefficient, and the density after culture is generally 1.0g L -1 the following
Cultivating bait microalgae in a closed bioreactor is a new type of culture. Although the culture density has increased, its maximum dry weight does not exceed 30g L -1 , and this method requires a lot of manpower and material resources, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Table 1 The cultivation effect of Phaeodactylum tricornutum

[0025]

[0026] The conditions of the foregoing examples are suitable conditions for the cultivation of common bait microalgae, and the density of the Phaeodactylum tricornutum seed liquid is 9.00×10 6 cells mL -1 , the volume ratio of the pretreated f / 2 culture solution to the added algal seed solution is 100:1. As shown in Table 1, the microalgae culture ecological regulator 8g L -1 , the obtained Phaeodactylum tricornutum product had the highest density (2.25×10 7 cells mL -1 ), compared with the control group in the same period, the culture density increased by 84.41%. In this example, the added microalgae culture eco-regulator can significantly promote the growth of Phaeodactylum tricornutum.

Embodiment 2

[0028] The culture effect of table 2 subcardioid algae

[0029]

[0030] The conditions of the foregoing examples are suitable conditions for the cultivation of common bait microalgae, and the density of the hypocardioid algae seed liquid is 1.33 × 10 6 cells mL -1 , the volume ratio of the pretreated f / 2 culture solution to the added algal seed solution is 100:1. As shown in table 2, the microalgae cultivation eco-regulator 8g L -1 , the density of the P. 6 cells mL -1 ), compared with the control group in the same period, the culture density increased by 24.79%. In this example, the added microalgae cultivation eco-regulator can significantly promote the growth of Phymina subcordiformis.

Embodiment 3

[0032] The culture effect of table 3 Isochrysis globosa

[0033]

control group

Example 1

Example 2

Example 3

Example 4

Ecological regulator for microalgae cultivation (g L -1 )

0

4

8

16

32

Final culture density (10 4 cells mL -1 )

136.58

273.75

337.25

421.25

319.58

Percentage increase in culture density (%)

0

100.43

146.92

208.43

133.99

Culture time (d)

8

8

8

8

8

[0034] The condition of above-mentioned embodiment is the suitable condition of common bait microalgae cultivation, and the density of dinoflagellate algae seed liquid is 1.33 * 10 6 cells mL -1 , the volume ratio of the pretreated f / 2 culture solution to the added algal seed solution is 100:1. As shown in table 3, the microalgae cultivation eco-regulator 16g L -1 , the obtained Isochrysis globosa product had the highest density (4.21×10 6 cells mL -1 ), compared with the control group ...

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Abstract

The invention discloses a microalga culture ecological preparation and an application method thereof, belonging to the technical field of alga culture. The microalga culture ecological preparation consists of oyster shell powder and magnesium hydroxide, wherein the magnesium hydroxide is prepared from bittern; the weight ratio of the oyster shell powder to the magnesium hydroxide is (1:2)-(2:1); and the microalga culture ecological preparation is in a granular form. In the invention, the added microalga ecological preparation can remarkably change the carbonate system in the culture water body, a carbon source is continuously provided for bait microalgae, and a constant relatively-high specific growth rate is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to an ecological regulator for microalgae cultivation and a using method thereof. Background technique [0002] Microalgae (Microalgae) is a general term for a group of small, low-level single-celled plants capable of photosynthesis. Its cells are rich in amino acids, polyunsaturated fatty acids, vitamins and other nutritional and biologically active substances, which can be widely used in many fields such as human health food, aquatic bait and animal feed, and have important economic value and social benefits . As fresh bait for aquatic animal larvae and nutrient-enhanced food for secondary bait organisms, microalgae in aquaculture bait have comprehensive nutritional components, which are significantly superior to those in terms of promoting larval growth, improving survival rate, and meeting the nutritional and growth needs of special stages of seedling cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 方建光姜娓娓樊海英李锋杜美荣房景辉
Owner 荣成恒顺海洋生物科技有限公司
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