Preparation method of phenylpropanoid compound

A compound, phenylpropanoid technology, applied in the field of medicine, can solve problems such as complex biological effects

Active Publication Date: 2016-05-25
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many target cells for IL-6, including macrophages, liver cells, resting T...

Method used

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  • Preparation method of phenylpropanoid compound
  • Preparation method of phenylpropanoid compound
  • Preparation method of phenylpropanoid compound

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Embodiment 1

[0057] The preparation of embodiment 1 phenylpropanoid compound

[0058] This embodiment provides a preparation method of phenylpropanoid compounds shown in formula (I), comprising the following steps:

[0059] S1. Take 50 kg of big-leaved jackfruit, use the root as raw material, dry it, and cut it into small pieces. Reflux extraction with 8 times the amount of 60% ethanol for 3 times, each time for 2 hours, combine the extracts, concentrate until there is no alcohol smell, and obtain the extract for later use;

[0060] S2. Dissolve the extract concentrated in step S1 in 10L of water, and use D101 macroporous adsorption resin column to elute it. The eluent is water, and the eluent is eluted for 3 column volumes. The eluate is collected and named as MM -1, standby;

[0061] S3. The fraction MM-1 collected in step S2 is eluted with reverse-phase ODS column chromatography, the eluent is methanol-water system, and its volume ratio is 25:75, and 18 column volumes are eluted, acco...

Embodiment 2

[0064] The preparation of embodiment 2 phenylpropanoid compounds

[0065] This embodiment provides a preparation method of phenylpropanoid compounds shown in formula (I), comprising the following steps:

[0066] S1. Take 40 kg of big-leaved jackfruit, use the root as raw material, dry it, and cut it into small pieces. Reflux extraction with 6 times the amount of 50% ethanol for 2 times, each time for 1 hour, combine the extracts, concentrate until there is no alcohol smell, and obtain the extract for later use;

[0067] S2. Dissolve the concentrated extract in step S1 in 5L of water, and use D101 macroporous adsorption resin column to elute it. The eluent is ethanol and water with a volume ratio of 15:85, and eluted for 3 column volumes , collect the eluate, name it MM-1, and set aside;

[0068] S3. The fraction MM-1 collected in step S2 is eluted with reverse-phase ODS column chromatography, the eluent is methanol-water system, and its volume ratio is 20:80, and 18 column v...

Embodiment 3

[0071] The preparation of embodiment 3 phenylpropanoid compounds

[0072] This embodiment provides a preparation method of phenylpropanoid compounds shown in formula (I), comprising the following steps:

[0073] S1. Take 60 kg of big-leaved jackfruit, use the root as raw material, dry it, and cut it into small pieces. 7 times the amount of 70% ethanol was refluxed and extracted 4 times, each time for 3 hours, the extracts were combined, concentrated until there was no alcohol smell, and the extract was obtained for later use;

[0074] S2. Dissolve the concentrated extract in step S1 in 8L of water, and use a D101 macroporous adsorption resin column to elute it. The eluent is ethanol and water with a volume ratio of 10:90, and eluted for 3 column volumes , collect the eluate, name it MM-1, and set aside;

[0075] S3. The fraction MM-1 collected in step S2 is eluted with reversed-phase ODS column chromatography, the eluent is methanol-water system, its volume ratio is 30:70, a...

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Abstract

The invention relates to the technical field of medicine and discloses a preparation method of a phenylpropanoid compound. The invention particularly relates to a method for separating a phenylpropanoid compound from dried root of flemingia macrophylla, and the phenylpropanoid compound is prepared by steps of solvent extraction, column chromatography separation and preparation of liquid-phase separation for purification. The phenylpropanoid compound obtained by the method disclosed by the invention is reported for the first time and has high purity. Proven by experiments, the obtained new phenylpropanoid compound has an effects on inhibiting the content of cell inflammatory factor NO, inhibiting expression of cell inflammatory factors IL-1 beta and IL-6 and inhibiting hydroxyl free radical (-OH), thereby having anti-inflammatory and oxidation resisting activities and benefiting treatment of cervicitis, endometritis, pelvic inflammation, mastitis, faucitis and/or arthritis and various inflammatory diseases. The phenylpropanoid compound can be developed into a new drug. The structural formula of the phenylpropanoid compound disclosed by the invention is as shown in formula (I) in the specification.

Description

technical field [0001] The invention relates to the technical field of medicine, and more specifically, to a preparation method of a new phenylpropanoid compound. Background technique [0002] The components extracted and separated from natural medicines have various structures and significant activities. Separation, purification, structural modification, transformation and total synthesis of the components have always been a main idea in the development of new drugs. [0003] TNF-α: It is a cytokine that can directly kill tumor cells without obvious toxicity to normal cells. It is one of the biologically active factors with the strongest direct tumor killing effect found so far, but its toxic and side effects are also very serious. [0004] IL-1β: At local low concentrations, it can co-stimulate the activation of APC and T cells, promote the proliferation of B cells and secrete antibodies, and perform immune regulation. Endocrine effects when produced in large quantities: ...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08A61P29/00A61P19/02A61P11/04A61P15/14A61P15/00
CPCA61K9/0019A61K9/08C07H1/08C07H15/203
Inventor 彭开锋张鹏伍实花林丽美夏博候佘娜张英帅
Owner ZHUZHOU QIANJIN PHARMA
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