Separation and purification method for lactococcus acidophilus antibacterial peptide
A Lactobacillus acidophilus, separation and purification technology, applied in the field of separation and purification of antimicrobial peptides, can solve problems such as protein denaturation
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[0015] The content of the present invention will be further described in detail below.
[0016] A method for separating and purifying Lactobacillus acidophilus antimicrobial peptides, comprising the following steps: (1) Solid fermentation of Lactobacillus acidophilus to obtain a Lactobacillus acidophilus fermentation product; (2) Dissolving the Lactobacillus acidophilus fermentation product in water, Take the supernatant and pass through multiple ultrafiltration membranes of different specifications to obtain components with different molecular weights; (3) freeze-dry the supernatant of each component, and measure the antibacterial activity of each component; (4) through coagulation Gel column chromatography obtained high-purity Lactobacillus acidophilus antimicrobial peptide; (5) Thermal stability test of Lactobacillus acidophilus antibacterial peptide. Further preferably, the fermentation temperature in step (1) is 36° C. to 37° C., the anaerobic fermentation is carried out,...
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