Bacillus wettable powder containing brassinolide and preparation method thereof
A technology of brassinolide and wettable powder, which is applied in the field of microbial fertilizers and biological pesticides, can solve problems such as poor product quality stability, backward production technology, and impact on application effects, and achieve long shelf life of spores, stable production technology, The effect of promoting crop growth
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Embodiment 1
[0026] A bacillus wettable powder containing brassinolide, the best components by weight are: 0.005 parts of brassinolide, 150 parts of Bacillus amyloliquefaciens bacteria liquid, emulsifier: nonylphenol poly 0.05 parts of oxyethylene ether, solvent: 0.75 parts of ethanol, spore protection agent: 5 parts of skimmed milk powder, 2 parts of methyl cellulose, dispersant: 3 parts of D425, wetting agent: 5 parts of sodium dodecylbenzenesulfonate, carrier: 70 parts of diatomaceous earth;
[0027] The preparation of the bacillus wettable powder containing brassinolide comprises the following steps:
[0028] 1. Preparation of Bacillus Bacteria Liquid
[0029] Take out the preservation tube of Bacillus amyloliquefaciens, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated into t...
Embodiment 2
[0034] A Bacillus wettable powder containing brassinolide The components of the wettable powder are in parts by weight: 0.002 parts of brassinolide, 120 parts of Bacillus subtilis bacterial liquid, emulsifier: nonylphenol 0.02 parts of polyoxyethylene ether, solvent: 0.3 parts of ethanol, spore protection agent: 3 parts of skimmed milk powder, 2 parts of methyl cellulose, dispersant: 3 parts of D425, wetting agent: 5 parts of sodium dodecylbenzenesulfonate, carrier : 75 parts of diatomite;
[0035] The preparation of the bacillus wettable powder containing brassinolide comprises the following steps:
[0036] 1. Preparation of Bacillus Bacteria Liquid
[0037] Take out the preservation tube of Bacillus subtilis, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated into th...
example 3
[0041] Example 3 Utilizes the test effect of the bacillus wettable powder containing brassinolide produced by example 1 in the paddy field
[0042] 1. The test was selected to be carried out in rice high-yielding areas;
[0043] 2. The test site is selected in the responsible field of a farmer in Longzhu Village, Tantou Town, Longhui County, Hunan Province;
[0044] 3. Test method: Drug usage and dosage: Test group: Dilute Bacillus amyloliquefaciens wettable powder containing brassinolide 1000 times in rice seedlings with two leaves and one heart to four leaves and one heart stage, 5-7 days after transplanting During the greening stage and the heading stage of rice opening, choose sunny days to apply pesticides. Bacillus group: Dilute the same amount of Bacillus amyloliquefaciens 1000 times and use it at the same time; Brassinolide group: Dilute the same amount of Brassinolide 1000 times for use; Blank control group: Spray the same amount of water . Each group uses an area ...
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Abstract
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