Multiple compound microbial liquid fertilizer, preparation method and applications thereof
A compound microbial and liquid fertilizer technology, which is applied in the field of "all-in-one" compound microbial liquid fertilizer and its preparation, can solve the problems of insufficient application area and dosage, inconvenient mechanized application, and low efficiency of biological bacterial fertilizer, etc., to improve crop quality , Protect your health, improve the effect of water retention and fertility
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Embodiment 1
[0031] 1. Supply of amino acid solution
[0032] Using sulfuric acid with a concentration of 5mol / L, acidolysis the lean meat of sick and dead pigs at 80°C for 6 hours to obtain an amino acid solution; the mass-volume ratio of lean meat to sulfuric acid is 1:1.5.
[0033] 2. Production of functional bacterial agents
[0034]The rhizosphere growth-promoting Bacillus amyloliquefaciens SQR-9 with the preservation number of CGMCC NO.5808 was inoculated into the LB culture solution, and the liquid fermentation production was carried out. ~180 rpm, spores are formed in the middle and late fermentation, so that the amount of bacteria or spores in the fermentation broth is ≥1×10 9 CFU / mL. The preparation method of the LB culture medium used is: take 1000 mL as a column, 5 g of yeast, 10 g of peptone, and 10 g of sodium chloride, dilute to 1000 mL with deionized water, stir evenly, adjust the pH value to 7.0-7.6, and sterilize at 121 °C for 20 min.
[0035] 3. Proportion generation ...
Embodiment 2
[0042] Field test
[0043] 1. Field effect of "full element" compound microbial liquid fertilizer on corn
[0044] This experiment was carried out in Hai'an County, Nantong City, Jiangsu Province. The experiment consisted of three treatments: treatment 1 was a blank control, no liquid fertilizer was applied; treatment 2 was an amino acid liquid fertilizer (urea 100g / L+dihydrogen phosphate) without Bacillus amyloliquefaciens. Potassium 50g / L+amino acid 200mL / L+molasses 200mL / L+deionized water 500mL / L); treatment 3 is the research and development product "Quanyuan" compound microbial liquid fertilizer (urea 100g / L+ dipotassium hydrogen phosphate 50g / L+ amino acid 200mL / L+ Molasses 200mL / L + deionized water 500mL / L + 10% Bacillus amyloliquefaciens SQR-9 fermentation broth), each treatment has 3 plots, plot length 5.25m, width 3m, area 15.75m 2 , the test variety was Suyu No. 29, and all treatment base fertilizers were uniformly fertilized according to the local fertilization met...
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