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Preparation method of anti-fatigue cordyceps militaris Hami melon ferment

A technology of Cordyceps militaris and cantaloupe, which is applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems such as the inability to meet the normal needs of people and the low enzyme content.

Inactive Publication Date: 2016-05-04
沈阳园康天然生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the enzyme content in daily food is too low to meet people's normal needs

Method used

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  • Preparation method of anti-fatigue cordyceps militaris Hami melon ferment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Step 1: Prepare the yeast seed solution:

[0022] Seed liquid tank expansion medium: YPD liquid medium, its mass percentage is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, the rest is water, put YPD liquid medium into 0.5M 3 In the seed tank, sterilize at 115°C for 30 minutes; under aseptic conditions, dissolve 1.5 kg of dry yeast powder in 3 times aseptic water to form a bacterial liquid, put the bacterial liquid into a serum bottle and Inoculate to 0.5M 3 The seed tank is fed with sterile compressed air at 28°C, the ventilation rate is 0.2 cubic meters per minute, and cultivated for 20 hours. When the bacteria grow to the end of the logarithmic phase, the cultivation is stopped. Repeat the above steps to obtain the required quality of yeast Seed liquid of bacterial liquid;

[0023] Step 2: Prepare lactic acid bacteria seed solution:

[0024] Seed liquid tank expansion medium: malt compound juice enrichment medium, its mass percentage is 10% wort, 0.5% soybean...

Embodiment 2

[0032] Step 1: Prepare the yeast seed solution:

[0033] Seed liquid tank expansion medium: YPD liquid medium, its mass percentage is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, the rest is water, put YPD liquid medium into 0.5M 3 In the seed tank, sterilize under high temperature at 115°C for 50 minutes; under aseptic conditions, dissolve 1.5 kg of dry yeast powder in 3 times aseptic water to form a bacterial liquid, put the bacterial liquid into a serum bottle and Inoculate to 0.5M 3 The seed tank is fed with sterile compressed air at 30°C, the ventilation rate is 0.3 cubic meters per minute, cultivated for 30 hours, and the culture is stopped when the bacteria grow to the end of the logarithmic phase, and the yeast of the required quality can be obtained by repeating the above steps Seed liquid of bacterial liquid;

[0034] Step 2: Prepare lactic acid bacteria seed solution:

[0035] Seed liquid tank expansion medium: malt compound juice enrichment medium, its mas...

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Abstract

The invention provides a preparation method of anti-fatigue cordyceps militaris Hami melon ferment. The preparation method comprises the following steps: step one, preparing a yeast strain liquid; step two, preparing a lactobacillus strain liquid; step three, culturing fermentation broth; step four, subjecting the fermentation broth to a centrifugation treatment to obtain an original liquid of ferment; step five, mixing a cordyceps militaris extract and the original liquid of ferment obtained in the step four according to a mass ratio of 33:1000, making the mixture stay in an environment with a temperature of 115 DEG C for 30 to 50 minutes so as to kill the bacteria, and finally filling the ferment into different containers.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, in particular to a preparation method of anti-fatigue Cordyceps militaris cantaloupe enzyme. Background technique [0002] Enzyme, also known as "enzyme", is a polymer substance with biocatalytic function. Almost all cellular processes require the participation of enzymes. In modern society, with the acceleration of people's life rhythm and the intensification of environmental pollution, the content of enzymes in people's bodies is decreasing day by day, which in turn reduces the body's immunity and metabolic rate, and greatly increases the chance of people getting sick. At present, the enzyme content in daily food is too low to meet people's normal needs. In order to meet the diversity, balance and easy absorption of modern people's nutritional needs, the production of high-efficiency enzyme products is imminent. Contents of the invention [0003] In order to overcome the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L31/00A01G1/04
CPCA01G18/00A23V2002/00
Inventor 不公告发明人
Owner 沈阳园康天然生物科技有限公司
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