Diabetes-coronary heart disease combined animal model building method based on ZDF rat

A technology of animal model and establishment method, which is applied in animal husbandry and other fields, can solve the problems of long model cycle and low success rate, and achieve the effect of short cycle and high molding rate

Inactive Publication Date: 2016-05-04
朱超
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it takes a long time to construct a rat model of diabetes complicated with coronary heart disease, and the success rate is low

Method used

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  • Diabetes-coronary heart disease combined animal model building method based on ZDF rat
  • Diabetes-coronary heart disease combined animal model building method based on ZDF rat
  • Diabetes-coronary heart disease combined animal model building method based on ZDF rat

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Experimental program
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Effect test

Embodiment 1

[0025] 1. Materials and Methods

[0026] 1.1 Main reagents, materials and instruments

[0027] Xiangyi H2050R high-pressure refrigerated centrifuge (Benxi Testejie Biotechnology Co., Ltd.), ThermoFisher enzyme immunoassay analyzer (Tianshi Medical Supplies Manufacturing Institute, Haidian District, Beijing), creatine kinase (CK) kit, creatine kinase isowork The enzyme (CK-MB) kits were all from Nanjing Ampere Chemical Technology Co., Ltd., isoproterenol hydrochloride injection (ISO, specification: 1mg / 2mL, Shanghai Hefeng Pharmaceutical Co., Ltd.).

[0028] 1.2 Experimental animals

[0029] ZDF male rats, 8 weeks old, SPF grade, including control rats (without diabetes, body weight 220-240 g) and model rats (with diabetes, body weight 330-350 g), provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. . Culture conditions: room temperature (20±2)°C, humidity (50±20)%. 2. Experimental method

[0030] 2.1 Rat grouping and model making

[0031] After 2 we...

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Abstract

The invention discloses a diabetes-coronary heart disease combined animal model building method based on a ZDF rat; the method comprises the following steps: adaptively feeding the ZDF male rat with diabetes for 2 weeks; injecting isoprenaline hydrochloride solution everyday in a hypodermic manner for 10 days, wherein the injection capacity is 1mg.kg-1; detecting serum creatine jubase (CK) and creatine jubase isozyme (CK-MB) activity, observing tissue form changes, and evaluating the model. The method uses the ZDF rat, and combines continuous administration with high fat feeding so as to form the diabetes-coronary heart disease combined animal model; the method is short in period, and high in modeling rate.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for establishing an animal model of diabetes complicated with coronary heart disease based on ZDF rats. Background technique [0002] Diabetes changes the function of various cells, including vascular endothelial cells, smooth muscle cells and platelet cells, which can lead to coronary atherosclerosis and plaque formation in blood vessels. Moreover, coronary artery disease in patients with diabetes mellitus and coronary heart disease is more serious, manifested in a wider range of lesions, often multi-vessel involvement, each vessel is diffusely involved in multiple places; most coronary arteries are severely stenotic, and suffer from diabetes The longer the time, the higher the degree of stenosis. Although diabetic patients with coronary heart disease have severe coronary artery disease, diabetic patients often have neuropathy and feel less pain, so they often do...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 朱超朱莹莹张志芹周开文曾强成沈亮
Owner 朱超
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