Production method of composite microbial agent for treatment of refractory wastewater
A composite microbial inoculum and a technology for composite microbial bacteria are applied in the field of production of composite microbial inoculants for refractory wastewater treatment, and achieve the effects of low production cost, good cultivation effect and low price.
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Embodiment 1
[0024] Embodiment 1, a kind of production method of refractory waste water treatment composite microbial bacterial agent, comprises the following steps:
[0025] (1) Medium preparation: add corn steep liquor, molasses (weight ratio 2:1) or other extractive organic carbon sources to the basic medium, so that the TOC concentration of the medium is 500mg / L;
[0026] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 1% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;
[0027] (3) Bacteria culture: control dissolved oxygen 0.2mg / L, microbial load 0.1kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure the TOC removal of the medium by the complex microbial flora The rate is not less than 80%;
[0028] (4) Scale-up culture: Carry out ...
Embodiment 2
[0029] Embodiment 2, a kind of production method of refractory waste water treatment composite microbial bacterial agent, comprises the following steps:
[0030] (1) Medium preparation: Add corn steep liquor, molasses (weight ratio 1:2) or other extractive organic carbon sources to the basic medium, so that the TOC concentration of the medium is 5000 mg / L;
[0031] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 10% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;
[0032] (3) Bacteria culture: control dissolved oxygen 2.0mg / L, microbial load 0.5kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure that the complex microbial flora removes TOC from the medium The rate is not less than 80%;
[0033] (4) Scale-up culture: Carry o...
Embodiment 3
[0034] Embodiment 3, a kind of production method of refractory wastewater treatment composite microbial bacterial agent, comprises the following steps:
[0035] (1) Medium preparation: Add corn steep liquor, molasses or other extractive organic carbon sources to the basal medium so that the TOC concentration of the medium is 3000 mg / L;
[0036] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 5% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;
[0037] (3) Bacteria culture: control dissolved oxygen 1.0mg / L, microbial load 0.3kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure that the complex microbial flora removes TOC from the medium The rate is not less than 80%;
[0038] (4) Scale-up culture: Carry out step-by-step scale-...
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