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A method of reducing the accumulation of cadmium in honeysuckle and increasing its yield by using Glomus terrescens

A technology of Glomus terrestrialis and honeysuckle, which is applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of reducing the accumulation of cadmium in honeysuckle, achieve the improvement of mineral nutrition status, Secondary pollution, good effect

Inactive Publication Date: 2019-05-28
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no report on the use of mycorrhizal fungi to reduce the accumulation of cadmium in honeysuckle

Method used

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  • A method of reducing the accumulation of cadmium in honeysuckle and increasing its yield by using Glomus terrescens
  • A method of reducing the accumulation of cadmium in honeysuckle and increasing its yield by using Glomus terrescens
  • A method of reducing the accumulation of cadmium in honeysuckle and increasing its yield by using Glomus terrescens

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Experimental program
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Effect test

Embodiment 1

[0045] 1. Propagation and culture of test strains and preparation of bacterial agents

[0046] Glomus versiforme (Gv for short) BGC GD01C provided by Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0047] After using corn (Zea mays) as a host plant for potted propagation, the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil is used as an inoculant. The specific process is as follows:

[0048] Fill the sterilized sand (according to the weight ratio, sand:soil=1:1) culture medium to 4 / 5 of the plastic basin. According to the inoculum amount of 4% (w:w), the Glomus terrescens inoculant was evenly spread on the substrate as a thin layer. Then cover the sterilized substrate 2cm, water, sow 10-15 corn seeds / pot. Cover with 0.5cm sterilized substrate, and then move to the greenhouse for cultivation.

[0049] Harvest after 5 months of potting. When harvesting, first cut off t...

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Abstract

The invention discloses a method for reducing the honeysuckle cadmium accumulation and increasing the honeysuckle yield through glomus versiforme berch. The method utilizes the symbiotic cultivation of glomus versiforme berch and honeysuckle, and specifically includes the steps of harvesting a mixture, namely, a glomus versiforme berch fungicide, containing host plant root segments, fungal hyphae, mycorrhiza fungal spores and cultivation soil after propagation cultivation is conducted on glomus versiforme berch through host plants, inoculating the glomus versiforme berch fungicide in a culture medium, and cultivating honeysuckle. By means of the method, the growth of honeysuckle can be promoted, and yield is increased; more importantly, the cadmium concentration of aboveground parts including stems, leaves and flowers of honeysuckle and underground parts including roots can be reduced. An approach is provided for safely producing honeysuckle in cadmium-polluted soil, important significance is achieved for production and application of honeysuckle, and the method is good in effect, free of secondary pollution and low in cost. Meanwhile, a new concept and direction are provided for development and utilization of glomus versiforme berch.

Description

technical field [0001] The invention belongs to the technical field of heavy metal pollution restoration. More specifically, it relates to a method for reducing cadmium accumulation and increasing yield of honeysuckle by using Glomus terrescens. Background technique [0002] Due to human activities such as farmland sewage irrigation, solid waste application, mining and smelting, soil cadmium pollution is becoming more and more serious. Cadmium is highly mobile and highly toxic in soil, and is one of the most toxic inorganic pollutants in the environment. Cadmium enters the plant body after being absorbed by plants, and can be enriched into the human body through the food chain, seriously threatening agricultural production and human health. For large areas of moderately and lightly polluted cultivated land, there is currently no economically feasible technical means to completely remove heavy metals from soil. The contradiction between a large population and a small amoun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14
Inventor 靖元孝江秋云卓逢王宇涛李韶山
Owner SOUTH CHINA NORMAL UNIVERSITY
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