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Preparing method for aperture-controllable bacterial cellulose porous microspheres

A technology of bacterial cellulose and bacterial cellulose membrane is applied in the field of preparation of bacterial cellulose porous microspheres, which can solve the problem of difficulty in preparing porous microspheres, and achieve the effect of wide application prospects.

Active Publication Date: 2016-04-13
陕西启悦医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial cellulose is insoluble in general solvents, and it is difficult to prepare porous microspheres by general methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Preparation of bacterial cellulose

[0029] First, inoculate Gluconacetobacter xylinus, a microbial strain with bacterial cellulose production capacity, into an activated medium with a pH value of 5.5, and culture it once for 30 hours at 30°C to obtain a cultured strain. The cultured strains were inoculated again in the activation medium, and cultured for 30 hours at 30°C to obtain activated strains; wherein, the activation medium contained 40 g / L of sucrose, 12 g / L of beef extract, and 4 g / L of disodium hydrogen phosphate. L, citric acid 0.9g / L, ethanol 9g / L, agar 18g / L; inoculate the activated bacteria in the expansion culture medium with a pH value of 5.5, shake at a temperature of 30°C and a rotation speed of 200rpm Cultivate for 18 hours to obtain seed liquid; wherein, the expansion culture medium contains 45 g / L of sucrose, 12 g / L of beef extract, 4.2 g / L of disodium hydrogen phosphate, 1.0 g / L of citric acid, and 9 g / L of ethanol; Inoculate the seed liquid in...

Embodiment 2

[0036] 1) Preparation of bacterial cellulose

[0037]Firstly, inoculate Acetobacter xylinum, a microbial strain with bacterial cellulose production capacity, into an activated medium with a pH value of 6.0, and culture it once for 32 hours at 28°C to obtain a cultured strain. Re-inoculated in the activation medium, and cultured for 32 hours at 28°C to obtain activated strains; wherein, the activation medium contained 50 g / L of sucrose, 10 g / L of beef extract, and 4.5 g / L of disodium hydrogen phosphate , citric acid 0.8g / L, ethanol 10g / L, agar 16g / L; inoculate the activated strains in the expansion culture medium with a pH value of 6.0, and shake the culture at a temperature of 28°C and a rotation speed of 180rpm 22h, to obtain the seed solution; wherein, the expansion medium contains sucrose 40g / L, beef extract 15g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.8g / L, ethanol 8g / L; Inoculate 3 mL of inoculum per 100 mL, inoculate the seed solution into the bacterial cell...

Embodiment 3

[0044] 1) Preparation of bacterial cellulose

[0045] First, inoculate Gluconacetobacter xylinus, a microbial strain with bacterial cellulose production capacity, into an activated medium with a pH value of 5.8, and culture it once at 32°C for 28 hours to obtain a cultured strain. The cultured strains were re-inoculated in the activation medium, and cultured for 28 hours at 32°C to obtain activated strains; the activation medium contained 45g / L of sucrose, 13g / L of beef extract, and 4.2g of disodium hydrogen phosphate / L, citric acid 1.0g / L, ethanol 8g / L, agar 20g / L; the activated bacteria were inoculated in the expansion culture medium with a pH value of 5.8, at a temperature of 32°C and a rotation speed of 160rpm, Shake culture for 20 hours to obtain seed liquid; wherein, the expansion culture medium contains 48g / L sucrose, 10g / L beef extract, 4g / L disodium hydrogen phosphate, 0.9g / L citric acid, and 9.5g / L ethanol ; According to the inoculation amount of every 100mL inocul...

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Abstract

The invention discloses aperture-controllable bacterial cellulose porous microspheres and a preparing method for the aperture-controllable bacterial cellulose porous microspheres. Bacterial cellulose is taken and dissolved in a solvent, a bacterial cellulose solution is obtained, template particles with different particle sizes are added in the bacterial cellulose solution, the mixture is put in an ultrasonic dispersing machine, the particles are evenly dispersed through ultrasonic treatment, and a dispersed solution is obtained; the dispersed solution and a surfactant are poured into an organic solvent for homogeneous emulsification, a w / o type bacterial cellulose emulsion is obtained, a precipitating agent is added in the bacterial cellulose emulsion and stirred, and microsphere suspension liquid is obtained; the microsphere suspension liquid is subjected to vacuum filtration, precipitate is collected and washed with n-butyl alcohol, acetone and acetone three times in sequence, the washed precipitate is then extracted with ethyl alcohol, precipitate is collected and subjected to frozen drying, and the aperture-controllable bacterial cellulose porous microspheres are obtained. The method has the advantages of being low in cost, simple in process, capable of facilitating industrialized production and the like, the stability and repeatability are good, the aperture of the bacterial cellulose porous microspheres ranges from 200 nanometers to 50 micrometers, and the bacterial cellulose porous microspheres are suitable for mass production.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a preparation method of bacterial cellulose porous microspheres with controllable pore diameters. Background technique [0002] As an important new material, porous microspheres have the characteristics of low density, large specific surface area, good stability and strong surface permeability, so they are widely used in chemistry, biology, materials science and other fields. For example, in the research and development of new drug preparations and the transformation of new dosage forms, it can be used as a new type of sustained / controlled release drug delivery carrier, which can protect drugs from damage, control drug release speed, prolong drug action time, reduce drug adverse reactions and reduce drug use. It can also be used for the targeted release of drugs in specific tissues and organs. In addition, due to its high-efficiency cell expansion and in...

Claims

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Application Information

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IPC IPC(8): C12P19/04
CPCC12P19/04
Inventor 张雯王学川任龙芳强涛涛李启明
Owner 陕西启悦医疗科技有限公司
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