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Cresol red colorimetric method for soil respiration measurement and measurement device

A soil respiration and measuring device technology, which is applied to the measurement of color/spectral characteristics, etc., can solve the problems of reagents that can only be used once, the accuracy of the measurement is not ideal, and the measurement range is limited, and the operation is convenient and fast, and the operation method is convenient and fast , the effect of simple structure

Inactive Publication Date: 2016-04-06
GUIZHOU UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing method uses NaOH and other highly corrosive strong alkalis to measure soil respiration. The safety of the test is poor, and the reagent can only be used once, which greatly increases the cost of the test, and the alkali solution absorption titration method is only used when the amount of carbon dioxide is 0.001g- The measurement between 0.002g is relatively accurate, and the measurement range is limited. The limitation is that the measurement accuracy is not ideal

Method used

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  • Cresol red colorimetric method for soil respiration measurement and measurement device
  • Cresol red colorimetric method for soil respiration measurement and measurement device
  • Cresol red colorimetric method for soil respiration measurement and measurement device

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Embodiment 1

[0029] Embodiment 1: the cresol red colorimetric method of a kind of soil respiration assay, its this method may further comprise the steps:

[0030] (1) Solvent preparation: use acid-base indicator cresol red, neutral salt and bicarbonate to prepare a mixed reagent, that is, cresol red reagent;

[0031] (2) Draw the quantitative cresol red reagent in step (1) into a plastic test tube, and cover it;

[0032] (3) Open the cover of the plastic test tube containing the cresol red reagent in step (2) and put it into the soil respiration measurement device, place it on the surface of the soil to be tested and seal it, start timing, take out the plastic test tube and cover it after 4-6 hours cover;

[0033] (4) Colorimetrically measure the absorbance of the cresyl red reagent after soil respiration in step (3) at a wavelength of 560 nm by an ultraviolet-visible spectrophotometer, and calculate the soil respiration intensity according to the standard curve and blank control. Intens...

Embodiment 2

[0047] Embodiment 2: a kind of soil respiration measuring device, described plastic test tube 1 and sealing cover one 2, described plastic test tube 1 is fixedly connected on the test tube rack 3, and described test tube rack 3 is inserted in the soil 4 to be tested, so The sealing cover-2 is covered on the surface of the soil 4 to be tested, the plastic test tube 1 is placed therein, and the soil sealing layer 5 is arranged around the lower end, the sealing cover-2 adopts a stainless steel cup, and the test tube holder 3 includes a socket that adopts a steel wire to be arranged in a spiral shape part 6 and the bottom side support part 7 that shrinks the steel wire helically into a socket part, the bottom side support part 7 extends the steel wire down around the center to form an insertion rod 8, and uses the steel wire to wrap the test tube to realize the support of the test tube, and the structure is simple. The equipment cost is low, the operation is convenient and fast, an...

Embodiment 3

[0048] Embodiment 3: a kind of soil respiration measuring device, described plastic test tube 1, to-be-tested soil container 10 and sealing cover two 9, described plastic test tube 1 is placed in the beaker 11, described to-be-tested soil container 10 and plastic test tube 1 Place it in the sealing cover 2 9 to keep it fully sealed, and the sealing cover 2 9 adopts a 14# ziplock bag.

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Abstract

The invention discloses a cresol red colorimetric method for soil respiration measurement and a measurement device. The measurement device comprises a plastic test tube containing a cresol red reagent and a sealing cover. The plastic test tube and soil to be measured are sealed in the sealing cover, the absorbancy of the cresol red reagent is measured with the colorimetric method under the wavelength of 560 nm after respiration, and soil respiration intensity is measured through a standard curve. By preparing the cresol red reagent from the acid-base indicator cresol red, neutral salt and bicarbonate to absorb CO2 in soil, cultured soil respiration intensity is measured, and field soil respiration intensity can also be measured; strong base such as NaOH with high corrosivity is not needed, the reagent can be used repeatedly, sensitivity and safety performance are high, special or expensive instruments are not needed, the operation method is convenient and quick, and test data are reliable and accurate. The measurement device also has the advantages of being simple in structure, low in price, and convenient and rapid to use and operate.

Description

technical field [0001] The invention belongs to the technical field of soil respiration measurement, in particular to a cresol red colorimetric method and a measurement device for soil respiration measurement. Background technique [0002] Soil respiration is the process by which soils produce and release carbon dioxide into the atmosphere. It includes three biological processes of microbial respiration, root respiration and soil animal respiration and an abiotic process: the chemical oxidation process of soil organic matter under high temperature conditions. Soil respiration is an important biological index to characterize soil quality and fertility. It reflects the intensity of soil biological activity and soil material metabolism, and can indicate the process and direction of ecosystem succession. It also directly affects atmospheric CO 2 Concentration and its changes, so the determination of soil respiration is of great significance in agricultural science, ecology and ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 段建军易香王小利焦克强郭振徐虎孙莎莎王星曾廷廷何颖楠吕艳超
Owner GUIZHOU UNIV
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