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Biotechnology for petroleum desulfurization and denitrification and application

A technology of petroleum and mixed microorganisms, which is applied in the field of biotechnology and environmental microorganisms, can solve the problems of limited types of substrate removal, insignificant removal effect, and difficult operation, and achieves easy reproduction, easy promotion, and simple cultivation conditions Effect

Active Publication Date: 2016-04-06
TIANJIN WEIYUE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is particularly important to remove nitrogen from petroleum, but the traditional biological nitrogen removal technology has various disadvantages that cannot be ignored: high cost, difficult operation, limited removal of substrate types, insignificant removal effect, and The existing research reports on denitrification microorganisms are almost all normal temperature microorganisms

Method used

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  • Biotechnology for petroleum desulfurization and denitrification and application
  • Biotechnology for petroleum desulfurization and denitrification and application
  • Biotechnology for petroleum desulfurization and denitrification and application

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Preparation of mixed microbial agent: Geobacillus thermoglucosidasius w-1 (Geobacillus thermoglucosidasius), which is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, has a preservation number of CGMCCNo.11780 and Mycobacterium goodiisp. , the strain was purchased from the American Type Culture Collection, its preservation number is ATCC No. 700504), New Apollo NS-E [DSM4359] (Thermotoganeapolitana, the strain was purchased from the American Type Culture Collection, its preservation number is ATCC No.49049), small thermoleophilic bacteria (Thermoleophilum minutum, the strain was purchased from the American Type Culture Collection, and its preservation number is ATCC No.35268) were inoculated at 1:1000 (v / v) into the cultured by autoclaving In the liquid, vortex stirring at 60°C and 150rpm, airtight (oxygenated) culture for 18 hours, the obtained bacterial liquid was mixed according to the ratio of parts by volume 1...

Embodiment 2

[0023] Activation and expanded cultivation of mixed microbial agents: Inoculate the mixed microbial agents at 1:1000 (v / v) into the culture medium sterilized by autoclaving, 50°C, 150rpm vortex stirring, airtight (oxygen) culture for 18 hours, the mixed microbial inoculum was obtained. The culture medium is: inorganic salt medium + 1% sucrose solution at final concentration.

Embodiment 3

[0025] (1) Mix the activated 5% mixed microbial agent, 45% high-sulfur diesel oil and the rest of the inorganic salt solution according to the weight percentage, vortex and stir at 60°C and greater than 50rpm, and seal the reaction for 24 hours;

[0026] (2) After the reaction is completed, remove the bacterial solution, and wash with pure water at 60°C and 150rpm with vortex stirring until there are no obvious impurities.

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Abstract

The invention discloses a biotechnology for petroleum desulfurization and denitrification and an application. A mixed microbial agent is prepared from activated geobacillus thermoglucosidasius w-1, mycobacterium goodiii, thermotoga neapolitana and small thermophilic and oil-degraded bacilli according to the volume ratio of 1:1:1:1. The mixed microbial agent reacts with high-sulfur diesel oil and inferior oil slurry in an organic salt solution, and the sulfur content and the nitrogen content of the two kinds of oil before and after the reaction are measured. A production method is simple in process, needed equipment is simple, cost is low, environment friendliness is achieved, produced fuel oil is good in quality, an ideal treatment effect is achieved for the oil, thermophilic microorganisms achieving the simultaneous desulfurization and denitrification function are adopted for achieving simultaneous desulfurization and denitrification of petroleum in the high-temperature environment, the biological desulfurization and denitrification efficiency of fuel oil can be remarkably improved, the environment pollution problems generated after use can be effectively solved, excellent characteristics of industrially-applied bacterial strains are achieved, and the bacterial strains have wide industrial application potential.

Description

technical field [0001] The invention relates to the fields of biotechnology and environmental microorganisms, in particular to a biotechnology and application of petroleum desulfurization and denitrification. Background technique [0002] There are sulfur and nitrogen compounds in petroleum products, and the use of this product containing sulfur and nitrogen compounds will pollute the environment and cause corrosion to equipment. Therefore, desulfurization and denitrification technologies are important research topics for the petrochemical industry and environmental protection. [0003] The deep desulfurization of petroleum and its products is an important issue that our country and even the world's petrochemical industry will face in the new century. Sulfur in petroleum mainly exists in the form of organic sulfides such as mercaptan, thioether, thiophene, benzothiophene (BT) and their alkyl substitutes and derivatives. These compounds produce a large amount of thiocyanide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/01C12R1/32
CPCC10G32/00C12N1/20
Inventor 王威朱琳
Owner TIANJIN WEIYUE BIOTECH CO LTD
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