A preparing method and applications of a novel Kefir culture
A kefir starter, a new type of technology, applied to the bacteria used in food preparation, applications, dairy products, etc., can solve the problems of poor fermentation effect, unstable fermentation, and difficult source of kefir grains
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Embodiment 1
[0024] Kluyveromyces marxianus (Kluyveromyces marxianus): China Industrial Microorganism Collection Center, the collection number is CICC1953;
[0025] Lactobacillus kefiri (Lactobacillus kefiri): the preservation number of China Industrial Microorganism Culture Collection Center is CICC20260;
[0026] Leuconostocmesenteroides: the preservation number of China Industrial Microorganism Culture Collection Center is CICC23516;
[0027] Acetobacter lovaniensis (Acetobacter lovaniensis): The preservation number of China Industrial Microorganism Culture Collection Center is CICC7011.
[0028] The specific operation process is as follows:
[0029] 1. Selection of strain ratio
[0030] Take 1mL of kefir fermentation broth for gradient dilution, and then draw 200μL of the diluted solution to apply to MRS solid medium (lactic acid bacteria selection medium), PDA solid medium (yeast selection medium), Elliker solid medium (acetic acid Bacteria selection medium: tryptone 20g / L, yeast p...
Embodiment 2
[0044] Kluyveromyces marxianus (Kluyveromyces marxianus): China Industrial Microorganism Collection Center, the collection number is CICC1953;
[0045] Lactobacillus kefiri (Lactobacillus kefiri): the preservation number of China Industrial Microorganism Culture Collection Center is CICC20260;
[0046] Leuconostocmesenteroides: the preservation number of China Industrial Microorganism Culture Collection Center is CICC23516;
[0047] Acetobacter lovaniensis (Acetobacter lovaniensis): The preservation number of China Industrial Microorganism Culture Collection Center is CICC7011
[0048] The specific operation process is as follows:
[0049] 1. Strain isolation
[0050] Take 1mL of kefir fermentation broth for gradient dilution, and then draw 200μL of the diluted solution to apply to MRS medium (lactic acid bacteria selection medium), PDA medium (yeast selection medium), Elliker medium (acetic acid bacteria selection medium) base), where PDA and Elliker medium were cultured i...
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