Multiple-gene helicobacter pylori detection system and kit and application thereof

Abstract

The invention relates to a multiple-gene helicobacter pylori detection system and a kit and application thereof. The helicobacter pylori detection system includes 21 pairs of primers respectively for a strain identification gene (16S rRNA), virulence genes (cagA, vacA-s1, vacA-s2, vacA-m1, vacA-m2, iceA1, iceA2, dupA, oipA and luxS), drug resistance genes (2143 locus of 23S rRNA, 148 locus of rdxA, 1777 locus of pbp1A and polymorphism of the 261 locus of gyrA) and quantitative analysis genes ureC and beta-globin of the helicobacter pylori. The multiple-gene helicobacter pylori detection system and the kit of the system do not need the steps including conventional isolated culture and the like, synchronous detection and analysis on strain identification, quantification, virulence and medicine resistance can be directly conducted on tissue samples in the same reaction system, the shortcomings of low flux, long consumed time, low detection rate and the like of a conventional detection method are overcome, a comprehensive, accurate and low-cost etiological diagnosis is clinically provided for the first time, and important references are provided for individualized diagnosis and accurate treatment of helicobacter pylori infection.

Description

technical field [0001] The invention relates to a multiplex gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, campylobacter bacterium that resides primarily in the human stomach. Helicobacter pylori infection is closely related to the occurrence and development of chronic atrophic gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer, so it has attracted extensive clinical attention. In 1994, the International Agency for Research on Cancer (IARC) has classified it as a human class I carcinogen, and it is the only bacterial pathogenic microorganism that has been classified as clearly carcinogenic to humans so far. Many reports suggest that Helicobacter pylori infection is related to coronary heart disease, rheumatoid disease, hepatobiliary disease, tuberculosis, pregnancy vomiting...

AI Technical Summary

Problems solved by technology

Its disadvantage is that it does not reflect current infection

5) Quantitative analysis: real-time PCR is commonly used, but this method has a single detection, low throughput, and high cost when analyzing multiple genes

6) Drug susceptibility test: This method is time-consuming, cumbersome to operate, and relies on the isolation and culture of strains

However, the current conventional detection methods have shortcomings such as long time, low sensitivity, high cost, low throughput, and in particular, the inability to detect multiple related factors at the same time.

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