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Method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation

A technology of microorganisms and cadaverine, applied in the field of genetically engineered bacteria, can solve the problems of no knockout or weakened cadaverine production, and achieve the effects of preventing degradation, improving utilization rate, and reducing production costs

Inactive Publication Date: 2016-03-30
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN200780006903.9 discloses the knockout, suppression or enhancement of a series of genes in the cadaverine synthesis pathway, but none of these documents and patents have found knockout or weakening of the lysine transporter gene lysE and the glucose-6-phosphate isomerase gene pgi can effectively increase the production of cadaverine

Method used

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  • Method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation
  • Method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation
  • Method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation

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Embodiment 1

[0032] A method for improving the production of cadaverine by microorganisms using sugar fermentation, in which the key regulatory sites for the synthesis of cadaverine by microorganisms are modified, for example, the key regulatory sites are the lysE site of the lysine transporter gene and / or the pgi site of the 6-phosphate glucose isomerase gene, or, the key regulatory site is a regulatory site that regulates the expression of the above-mentioned genes.

[0033] Preferably, the modification of the key regulatory sites for the synthesis of cadaverine by microorganisms is: knocking out the lysine transporter gene lysE and / or the glucose-6-phosphate isomerase gene pgi, and / or, by changing their promoters, ribosome binding sites, or genetic mutations to attenuate their expression or attenuate enzyme activity.

[0034] Preferably, the microorganism is Escherichia coli, Corynebacterium glutamicum, Haffney hive or their recombinant strains.

[0035] The method for improving the p...

Embodiment 2

[0057] A method for improving the production of cadaverine by microorganisms using sugar fermentation, by modifying key regulatory sites in the metabolic pathway of microorganisms to synthesize cadaverine.

[0058] Preferably, the key regulatory sites are the lysE site of the lysine transporter gene and the pgi site of the 6-phosphate glucose isomerase gene, or the regulatory sites of these two genes that regulate the gene expression of the above key regulatory sites point.

[0059] Preferably, the transformation is: knocking out the lysine transporter gene lysE and / or the glucose-6-phosphate isomerase gene pgi, and / or, by changing their promoters, ribosome binding sites or performing gene Mutations to attenuate their expression or attenuate enzyme activity.

[0060] Preferably, the microorganism is Escherichia coli, Corynebacterium glutamicum, Haffney hive or their recombinant strains.

[0061] The specific steps of the transformation are as follows:

[0062] By cloning th...

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Abstract

The invention relates to a method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation. According to the method, key regulation and control sites in a metabolic pathway for synthesizing cadaverine through microorganisms are modified, and preferably, the key regulation and control sites are lysine transport protein gene lysE site and / or D-glucose-6-phosphateketol-isomerase gene pgi site, or the key regulation and control sites are regulation and control sites for regulating and controlling expression of the above genes. The modification method for the regulation and control sites comprises deleting lysine transport protein gene lysE and / or D-glucose-6-phosphateketol-isomerase gene pgi, or weakening expression or enzyme activity of lysine transport protein gene lysE and / or D-glucose-6-phosphateketol-isomerase gene pgi, and therefore the yield of cadaverine is effectively improved, saccharides utilization rate is increased, and production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of genetically engineered bacteria, in particular to a method for improving the production of cadaverine by effectively improving the production of cadaverine by transforming the key regulatory sites in the metabolic pathway of microorganisms using sugars to ferment and synthesize cadaverine. The cadaverine method. Background technique [0002] Cadaverine (Cadaverine) is a polyamine, that is, 1,5-pentanediamine (pentamethylenediamine for short), which is produced by decarboxylation of lysine in organisms, and is a biologically active nitrogen-containing compound widely present in organisms. Alkaline, but also present in spoilage as a carnitine. Cadaverine is an important raw material for the synthesis of new materials polyamide-54 (condensed from cadaverine and succinic acid) and polyamide-56 (condensed from cadaverine and oxalic acid), and has important industrial applications. [0003] Currently, there a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N15/77C12R1/15
CPCC12P13/001C07K14/34C12N9/88C12N9/92C12N15/77C12Y401/01018C12Y503/01009
Inventor 黎明路福平唐奇
Owner TIANJIN UNIV OF SCI & TECH
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