Preparation method for sargassum confusum C.Ag. phlorotannins

Abstract

The invention provides a preparation method for sargassum confusum C.Ag. phlorotannins. The preparation method comprises the steps: evenly mixing dried sargassum confusum C.Ag. and a 40 vol% ethanol solution with the amount 20 times that of the dried sargassum confusum C.Ag., carrying out water bath extraction for 46 min at the temperature of 30 DEG C, centrifuging the extract liquid for 10 min at the speed of 4000 r / min, repeating extraction for 2 times, and thus obtaining a phlorotannins extract liquid. The method for optimizing the sargassum confusum C.Ag. phlorotannins best extraction conditions by a response surface method is used, and 3 independent variables comprising the ethanol volume fraction, the material-to-liquid ratio and the extraction time are set; screening is performed by using a Box-Benhnken response surface design principle; experimental design and result analysis are performed by using Design expert software; the exaction technology of the sargassum confusum C.Ag. phlorotannins improves the extraction rate of the phlorotannins, and plays an important role in industrialized production of the phlorotannins.

Description

technical field [0001] The invention relates to the field of functional food or biomedicine, in particular to a preparation method of eruption polyphenols. Background technique [0002] Sargassum confusum C.Ag. is a plant that grows in warm temperate waters and is rich in coastal resources in my country. Jellyfish is rich in bioactive substances and has high health care and medicinal value. Artemisia polysaccharides have toxic and side effects on leukemia cell lines and can inhibit the proliferation of tumor cells. Fucoidan is also one of the main active ingredients. Modern studies have shown that fucoidan has various physiological functions such as anti-tumor, anti-virus, anti-thrombosis, anti-oxidation, blood lipid regulation, anti-radiation and inhibition of complement activation. Our country is rich in Echinacea resources, but due to the late start of the research on the comprehensive development of Echinacea, most of the research on its biological activity is concentr...

AI Technical Summary

Problems solved by technology

However, several methods are not suitable for the drying of echinacea

Drying, air drying and microwave drying can easily cause the loss of polyphenols in sea spores; the drying speed of sea spunberries is slow due to the many microorganisms on the surface during the drying process; the freeze-drying has problems such as slow processing speed and high cost, and cannot Universal use

[0005] Microwave-assisted extraction and ultrasonic-assisted extraction can shorten the extraction time, the extraction rate is high, the operation is simple, the extraction structure is not easy to damage, but the cost is high and the energy consumption is large. The method commonly used to extract polyphenols is organic solvent extraction.

Factors that affect the extraction of polyphenols from Echinacea include solvent volume fraction, liquid-to-solid ratio, extraction temperature, extraction time, extraction times, etc. The pretreatment of raw materials also affects the extraction of polyphenols. However, the traditional experimental design is currently used Methods The extraction process of Polyphenols from Echinacea Fructus has many experiments, long time-consuming experiments, low extraction rate of polyphenols from Esperia sinensis, and large investment in manpower and material resources, which is not conducive to industrial production.

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