Suspension cell sphere immunofluorescent staining method and staining device
A technique for immunofluorescence staining and suspension cells, which is applied in the field of suspension cell sphere immunofluorescence staining methods and staining devices, can solve the problems of time-consuming and labor-consuming, prolonged antibody incubation time, and long-consuming time, and achieves saving labor and time, saving Experiment cost and the effect of improving the success rate
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[0052] An immunofluorescence staining method for suspension cell spheres, the staining method includes the following steps:
[0053] (I) Combine cell chamber 1 and staining kit 2, take the cell ball suspension and add it to cell chamber 1, let it stand, unplug staining kit 2, and discard the medium in staining kit 2;
[0054] (Ii) Reconstitute cell chamber 1 and staining kit 2. Add 200ul PBS to cell chamber 1 to wash the cell pellets, let stand for 5 minutes, remove staining kit 2, discard PBS in staining kit 2, and repeat the operation three times;
[0055] (Iii) Reconstitute cell chamber 1 and staining kit 2, add 100ul of paraformaldehyde with a mass concentration of 4% to cell chamber 1 and fix for 10 minutes, remove staining kit 2, and discard the liquid in staining kit 2;
[0056] (Iv) Reconstitute cell chamber 1 and staining kit 2. Add 200ul PBS to cell chamber 1 to wash the cell pellets, let stand for 5 minutes, remove staining kit 2, discard PBS in staining kit 2, and repeat th...
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