Preparation method for kitasamycin industrial production strains

Abstract

The invention discloses a preparation method for kitasamycin industrial production strains, which comprises the main steps: performing plate streaking on an original strain to prepare a single colony; transferring the single colony onto an inclined plane, culturing same for a proper time; then scraping spores on the inclined plane with an inoculation loop, and preparing a freeze-dried strain tube with a vacuum freeze-drying method; preparing a spore suspension by using the freeze-dried strain tube, coating the oblique plane with the spore suspension, culturing the spore suspension for a proper time; then scraping spores on the oblique plane with the inoculation loop to prepare a spore and milk suspension; subpackaging the spore and milk suspension and performing freezing preservation so as to prepare a spore and milk freezing tube; melting the spore and milk freezing tube, and transferring same to a seed culture medium, performing shake culture by bottle shaking for a proper time, and then transferring to a seed tank. The method overcome the defects of poor synchronization of inoculation production of inclined strain production, nonuniform growth cycle of seed bottles and frequency of batch number change. The preparation method disclosed by the invention is simple and convenient to operate, the inoculum age of strains and consistency of quality of seeds are improved, the batch number change of seeds is reduced, and the preparation method is very suitable for kitasamycin industrial production.

Description

technical field [0001] The invention relates to a method for preparing and cultivating strains of biological fermentation, in particular to a method for preparing strains for industrialized production of kitasamycin. Background technique [0002] Kitamycin is a macrolide antibiotic, which is an antibacterial drug. It has a strong antibacterial effect on Gram-positive bacteria and negative bacteria, Rickettsia, spirochetes and large viruses, and has a Cyclic lactone-resistant bacteria are also effective; although the antibacterial spectrum is similar to erythromycin, it does not induce drug resistance, and the bacteria develop resistance slowly. It is a good alternative to antibiotics and is widely used in penicillin-resistant grapes. Bacteria, Streptococcus, Streptococcus pneumoniae infection, at the same time, in Europe, America, Japan and other countries, kitasamycin is also often used as a feed additive, and it is also a type of drug that can be used as clearly stipulated...

AI Technical Summary

Problems solved by technology

At present, the preparation process of kitasomycin seeds generally adopts fresh slant spore inoculum culture in shake flasks and then cultures in seed tanks. Because the fresh slant spores are stored in the process, the spore viability will change, limited by the storage time, it is impossible to prepare too much at one time. Multiple slopes are reserved, the growth cycle of the seeds prepared by this process is unstable, and the batches are changed frequently, resulting in the instability of the fermentation level of kitasamycin

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