Pseudomonas aeruginosa for producing rhamnolipid biosurfactant with palm oil and application thereof

Abstract

Rhamnolipid is an anionic surfactant which is free of toxicity and pollution, biodegradable, high in selectivity and specificity and excellent in surface activity and has unique application prospects in multiple industrial fields such as medicine, food, cosmetics and environment protection. The invention provides Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 with the high rhamnolipid yield and the high conversion rate through screening. Related fermentation equipment is transformed according to the fermentation characteristic of Pseudomonas aeruginosa, and a series connection fermentation experiment of 300L fermentation tanks is realized. According to the production state of the producing strain and the characteristic of the equipment, the production process of producing rhamnolipid in a fermented mode through Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 is developed, and while the rhamnolipid yield is high, the liquid leakage problem caused by surface activity brought by a great deal of rhamnolipid can be controlled. By using the equipment and the fermentation process, when palm oil with the concentration of 75 g / L serves as a carbon source, the rhamnolipid yield reaches 60.6 g / l, and the conversion rate reaches 80% or more. Therefore, the strain and the production process have good industrial application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Pseudomonas aeruginosa ( Pseudomonasaeruginosa ) TIB-R02CGMCCNo9376 and the method for producing rhamnolipid by fermentation of palm oil by the bacteria. Background technique [0002] Biosurfactant is a kind of non-toxic, biodegradable, non-polluting, good selectivity and specificity compared with chemically synthesized surfactants. Uniformity, good biocompatibility, etc., have unique application prospects in many industrial fields such as medicine, food, cosmetics, and environmental protection. Vigorously developing the biosurfactant technology industry will successfully drive the overall development of petroleum, medicine, detergent and other related fields, promote social progress and economic development, enable my country to have independent intellectual property rights, and improve international competitiveness. [0003] Rhamnosyl esters are anionic bi...

AI Technical Summary

Problems solved by technology

Traditional defoaming methods such as feeding defoaming agent, mechanical defoaming and pressurized defoaming cannot achieve the effect of defoaming, resulting in a large amount of foam carrying medium to escape from the exhaust pipe, resulting in waste of medium and product loss, while increasing Due to the risk of bacterial contamination, the requirements for fermentation production cannot be met

Images