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Method for improving conversion efficiency of lignocellulose through combined treatment and method for efficiently preparing ethyl alcohol

A lignocellulose and combined treatment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsatisfactory and low conversion rate of enzymatic hydrolysis, so as to reduce the dosage, increase the conversion rate of sugar, The effect of simple and easy preprocessing

Inactive Publication Date: 2016-03-09
QILU UNIV OF TECH
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Problems solved by technology

[0007] However, when the above method is used in the pretreatment process of lignocellulose enzymatic hydrolysis, the enzymatic conversion rate is relatively low and cannot meet the relevant requirements.

Method used

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  • Method for improving conversion efficiency of lignocellulose through combined treatment and method for efficiently preparing ethyl alcohol
  • Method for improving conversion efficiency of lignocellulose through combined treatment and method for efficiently preparing ethyl alcohol
  • Method for improving conversion efficiency of lignocellulose through combined treatment and method for efficiently preparing ethyl alcohol

Examples

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Embodiment 1

[0044] A method for joint treatment to improve lignocellulose conversion efficiency, comprising steps as follows:

[0045] 1) Alkali preleaching: soak corn stalks in NaOH solution and keep warm at 80°C for 1 hour. The ratio of the mass of corn stalk to the volume of water is 5:100, unit: g / mL. The amount of NaOH added is 0.03gg / g corn stover. After preleaching, the insoluble corn stover was washed with 2 volumes of deionized water to remove all soluble matter. The liquid is removed by extrusion and the solid matter is retained, which is the corn stalk after pre-leaching.

[0046] 2) Basic H 2 o 2 Treatment: Combine the pre-soaked corn stover with H 2 o 2 mix, h 2 o 2 The addition amount is 0.025 g / g corn stalks, and the pH is adjusted to 11.7 with 5 mol / l NaOH, and placed in a shaker at 30° C. at 180 rpm for 24 hours. the basic H 2 o 2 Processed rice straw.

[0047] 3) Enzymatic hydrolysis: the basic H 2 o 2 The treated rice straw was adjusted to pH 5.2 with 72% ...

Embodiment 2

[0050] The method for improving lignocellulose conversion efficiency through joint treatment described in Example 1 is different in that the amount of NaOH added is 0.06 g / g corn stalks.

Embodiment 3

[0052] It is the same as the method for improving lignocellulose conversion efficiency by joint treatment described in Example 1, except that the amount of NaOH added is 0.08 g / g corn stalks.

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Abstract

The invention relates to a method for improving the conversion efficiency of lignocellulose through combined treatment and a method for efficiently preparing ethyl alcohol. The method for improving the conversion efficiency of the lignocellulose through combined treatment includes the steps that a lignocellulose raw material is pre-extracted through alkaline, then alkaline H2O2 treatment is conducted, and afterwards, enzyme hydrolysis is carried out. According to the treatment method, alkaline pre-extraction is carried out first, and then alkaline H2O2 treatment is conducted, so that the enzymolysis conversin rate of the lignocellulose is greatly increased, and the glucose conversion rate of the cellulose can be urged to be further increased. The pretreatment process is simple and easy to implement, and wastewater which is difficult to deal with can be avoided. The using amount of H2O2 in traditional alkaline hydrogen peroxide pretreatment is reduced, and meanwhile the H2O2 can be made to act on remaining lignin more effectively.

Description

technical field [0001] The invention relates to a method for improving lignocellulose conversion efficiency through combined treatment and a method for efficiently preparing ethanol, belonging to the technical field of conversion and utilization of biomass resources. Background technique [0002] Lignocellulose is the most abundant and cheap renewable resource on earth, and it contains huge biomass energy. It mainly comes from agricultural waste, forest waste and urban waste. With the depletion of fossil fuels and the deterioration of the environment, the conversion of lignocellulosic sugars into glucose and xylose, etc., and further use in the production of bio-based chemicals such as fuel ethanol and other energy products has attracted increasing attention. my country is a large agricultural country with abundant crop straw resources, with an annual output of nearly 800 million tons. However, for a long time, most of the straws have been discarded or burned, which not onl...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P7/10C12R1/865
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 刘同军
Owner QILU UNIV OF TECH
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