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Method for directed differentiation of induced pluripotent stem cells into hepatocytes and hepatocytes

A pluripotent stem cell and directional induction technology, which is applied in the field of directional induction and differentiation of induced pluripotent stem cells into liver cells, can solve the problems of poor coagulation function, insufficient number of autologous cells, and low proliferation activity, etc., achieving short induction period, Stable performance and high efficiency

Active Publication Date: 2019-06-14
GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN
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Problems solved by technology

However, autologous bone marrow MSCs also have certain limitations: such as poor coagulation function of patients affecting bone marrow collection, insufficient number of autologous cells and low proliferation activity in patients, thus limiting clinical application.

Method used

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  • Method for directed differentiation of induced pluripotent stem cells into hepatocytes and hepatocytes
  • Method for directed differentiation of induced pluripotent stem cells into hepatocytes and hepatocytes
  • Method for directed differentiation of induced pluripotent stem cells into hepatocytes and hepatocytes

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Embodiment Construction

[0034] The method for directional induction of induced pluripotent stem cells to differentiate into hepatocytes of the present invention will be further described in detail mainly in conjunction with the accompanying drawings and specific examples.

[0035] In one embodiment, the method for directed differentiation of induced pluripotent stem cells into hepatocytes comprises the following steps:

[0036] Step S110, providing or preparing induced pluripotent stem cells.

[0037] In this embodiment, the induced pluripotent stem cells are preferably introduced into human cells by virus infection, plasmid transfection, mRNA introduction, miRNA introduction or chemical molecule addition, such as into fibroblasts, etc., to induce human cell differentiation Induced pluripotent stem cells. Among them, the induction factors include OCT4, SOX2, NANOG and Lin28. The fibroblasts may be isolated adult, infant or fetal skin fibroblasts and the like. It can be understood that in other embod...

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Abstract

The invention relates to a method for differentiating an induced pluripotent stem cell into a hepatocyte through directed induction, and the hepatocyte thereof. The method is characterized in that different media are added in different stages of directed induction differentiation to carry out directed induction differentiation of the induced pluripotent stem cell. The change of the form of the cell can be momentarily observed in the operating process to ensure normal implementation of induction, so a required liver precursor cell can be fast and efficiently obtained through induction. The method for differentiating the induced pluripotent stem cell into the hepatocyte through directed induction adopts a culture system of feeder-free cells, the hepatocyte can be obtained only through adding an induction medium in a corresponding stage, and a detection result shows that the purity is high. The method for differentiating the induced pluripotent stem cell into the hepatocyte through directed induction has the advantages of short induction period, high efficiency, stable performances and mature functions of the obtained hepatocyte, and no matrix cell pollution.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to a method for directional induction and differentiation of induced pluripotent stem cells into hepatocytes and the hepatocytes. Background technique [0002] Allogeneic liver transplantation is considered to be the most effective method for the treatment of end-stage liver disease, and the long-term survival rate after transplantation can reach 70%-85%. However, the limited liver donors are far from meeting the clinical needs. According to reports, the mortality rate of patients waiting for liver transplantation is as high as 15% during the waiting period. Other current treatments for liver failure include extracorporeal artificial liver through membrane filtration or resin exchange, bioartificial liver using allogeneic or heterogeneous hepatocytes in vitro, and in vivo hepatocyte transplantation. [0003] In the 1970s, cell-based therapy attracted widespread attention, and human ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
CPCC12N5/067C12N2501/117C12N2501/18C12N2501/237C12N2501/602C12N2501/603C12N2501/605C12N2501/608C12N2501/999C12N2506/45
Inventor 孙懿林戈卢光琇
Owner GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN
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