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Preparation method of orderly nanofiber membrane based on bacterial cellulose

A technology of bacterial cellulose membrane and bacterial cellulose, which is applied in the field of preparation of ordered nanofiber membranes, can solve problems such as influence and lack of antibacterial properties, and achieve the effects of expanding application fields, easy operation, and superior antibacterial properties

Inactive Publication Date: 2016-03-09
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although bacterial cellulose has many excellent properties, it has no antibacterial properties, which affects its application and development in the fields of biomaterials such as medical antibacterial dressings and artificial skin.

Method used

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  • Preparation method of orderly nanofiber membrane based on bacterial cellulose
  • Preparation method of orderly nanofiber membrane based on bacterial cellulose
  • Preparation method of orderly nanofiber membrane based on bacterial cellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Prepare 100 mL of seed culture with yeast extract (5 g / L), glucose (5 g / L), potassium dihydrogen phosphate (1 g / L), magnesium sulfate heptahydrate (15 g / L) and peptone (5 g / L) respectively base and 200 mL of fermentation medium. Put the seed medium and fermentation medium into a sterilizing pot at a temperature of 121°C and a pressure of 0.1MPa, and sterilize for 20 minutes. After the culture medium is cooled, add ethanol (20mL / L). The activated Acetobacter xylinum was inoculated into the seed medium, and cultured in a shaker at 28° C. and 160 rpm / min for 24 hours. According to the inoculum amount of 6%, inoculate the seed culture solution into the fermentation medium and shake it well to mix it evenly, and then culture it statically at 28°C for 7 days to obtain the bacterial cellulose film. Rinse the residues on the surface of bacterial cellulose with deionized water, soak in 0.1M NaOH solution, heat and stir at 80°C for 1 h, then adjust the pH to 7.0 with acetic acid...

Embodiment 2

[0028] Prepare 100 mL of seeds with yeast extract (6 g / L), glucose (6 g / L), potassium dihydrogen phosphate (0.8 g / L), magnesium sulfate heptahydrate (18 g / L) and peptone (6 g / L) respectively medium and 200 mL of fermentation medium. Put the seed medium and fermentation medium into a sterilizing pot at a temperature of 121°C and a pressure of 0.1MPa, and sterilize for 20 minutes. After the culture medium is cooled, add ethanol (10mL / L). The activated Acetobacter xylinum was inoculated into the seed medium, and cultured in a shaker at 30° C. and 160 rpm / min for 24 hours. According to the inoculation amount of 8%, the seed culture solution was inoculated into the fermentation medium, shaken sufficiently to mix evenly, and then cultured statically at 30°C for 7 days to obtain the bacterial cellulose film. Rinse the residues on the surface of bacterial cellulose with deionized water, soak in 0.2M NaOH solution, heat and stir at 85°C for 1 h, then adjust the pH to 7.0 with acetic a...

Embodiment 3

[0030] Prepare 100 mL of seed culture with yeast extract (5 g / L), glucose (5 g / L), potassium dihydrogen phosphate (1 g / L), magnesium sulfate heptahydrate (15 g / L) and peptone (5 g / L) respectively base and 200 mL of fermentation medium. Put the seed medium and fermentation medium into a sterilizing pot at a temperature of 121°C and a pressure of 0.1MPa, and sterilize for 20 minutes. After the culture medium is cooled, add ethanol (20mL / L). The activated Acetobacter xylinum was inoculated into the seed medium, and cultured in a shaker at 32° C. and 160 rpm / min for 24 hours. According to the inoculation amount of 8%, the seed culture solution was inoculated into the fermentation medium, shaken sufficiently to mix evenly, and then cultured at 32°C for 10 days to obtain the bacterial cellulose film. Rinse the residues on the surface of bacterial cellulose with deionized water, soak in 0.15M NaOH solution, heat and stir at 75°C for 2h, then adjust the pH to 7.0 with acetic acid, an...

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Abstract

The invention discloses a preparation method of an orderly nanofiber membrane based on bacterial cellulose. The orderly nanofiber membrane is obtained by activating hydroxide radicals on surfaces of bacterial cellulose through a NaOH aqueous solution, conducting a reaction in a quaternary ammonium salt ethanol solution, and conducting absolute ethyl alcohol washing and vacuum drying on a sample. Grafting modification is conducted on bacterial cellulose through quaternary ammonium salt, fiber in a prepared bacterial cellulose membrane has a high order, performance of bacterial cellulose is kept, an excellent antibacterial property is achieved, and the orderly nanofiber membrane can be used as a biological material for being widely applied to the fields of medical antibacterial dressings, artificial skin and the like.

Description

technical field [0001] The invention relates to the technical field of surface modification, in particular to a method for preparing an ordered nanofiber membrane based on bacterial cellulose. Background technique [0002] With the rapid development of science and technology and the continuous improvement of living standards, people have higher and higher requirements for sanitary products, and antibacterial materials have received great attention. The use of long-acting antibacterial materials and products is a newly developed method to prevent harmful microbial infection. One, it can effectively avoid the abuse of soluble disinfectants or antibiotics. Quaternary ammonium salt is a kind of cationic surfactant, which has the characteristics of high efficiency, broad spectrum, strong bactericidal effect and low toxicity. It has a good bactericidal effect on Gram-negative bacteria, Gram-positive bacteria and fungi. Quaternary ammonium salt is an organic small molecule antibac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08L1/02C12P19/04C12R1/01
CPCC08J7/12C08J2301/02C12P19/04
Inventor 邵伟刘晖吴祭民王淑侠黄敏
Owner NANJING FORESTRY UNIV
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