Efficient in-vitro plant regeneration method of adult high-quality single-plant Xiaoqiao oriental cherry of cerasus lannesiana var. speciosa
A mature and excellent technology for cherry blossoms, applied in the directions of plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of difficulty in establishing a sterile line of reproduction, complicated rooting culture process, long subgeneration cycle, etc., and achieve high rooting rate. , the effect of short generation cycle and fast induction speed
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Embodiment 1
[0031] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:
[0032] (1) Collection of explants: At about 10 am when the weather is fine from March to May, cut half-grown, healthy and pest-free cherry trees from the mother plant of 'Xiao Qiao' (adult excellent tree over ten years old). The lignified shoots were taken back to the laboratory after moisturizing treatment, and the collected semi-lignified shoots were removed from the leaves, washed with pure water and stored as explants in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.
[0033] (2) Surface disinfection of explants: cut branches (explants) into about 4cm long axillary stem segments with leaves, wash them with sterile water on the ultra-clean workbench, and then use 0.5% Xinjier with mass frac...
Embodiment 2
[0043] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:
[0044] (1) Collection of explants: From March to May, at about 10 am when the weather is fine, cut semi-woody semi-woody trees that are healthy and free from diseases and insect pests from the mother plant of 'Xiaoqiao' cherry tree (an excellent tree more than ten years old). The new shoots were transformed, and after moisturizing treatment, they were taken back to the laboratory. The collected semi-lignified shoots were removed from the leaves, cleaned with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.
[0045] (2) Surface disinfection of explants: cut the branches (explants) into about 4cm long axillary stem segments with leaves, wash them...
Embodiment 3
[0055] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:
[0056] (1) Collection of explants: From March to May, at about 10 am when the weather is fine, cut semi-woody semi-woody trees that are healthy and free from diseases and insect pests from the mother plant of 'Xiaoqiao' cherry tree (an excellent tree more than ten years old). The new shoots were transformed, and after moisturizing treatment, they were taken back to the laboratory. The collected semi-lignified shoots were removed from the leaves, cleaned with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.
[0057] (2) Surface disinfection of explants: cut branches (explants) into about 4cm long axillary stem segments with leaves, wash them wit...
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