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Method for detecting nicotine and secondary alkaloids in nicotine liquid and main stream smoke of electronic cigarette

A GC-MS, mainstream smoke technology, applied in the determination of anonicotine and cotinine, pseudobasine, mesmin, nicotine, nornicotine

Inactive Publication Date: 2016-03-02
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no reports on the detection of nicotine and 5 minor alkaloids in the mainstream smoke of e-cigarettes

Method used

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  • Method for detecting nicotine and secondary alkaloids in nicotine liquid and main stream smoke of electronic cigarette
  • Method for detecting nicotine and secondary alkaloids in nicotine liquid and main stream smoke of electronic cigarette
  • Method for detecting nicotine and secondary alkaloids in nicotine liquid and main stream smoke of electronic cigarette

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0054] 1. Reagents and instruments:

[0055] Standard products: 2-methylquinoline, nicotine, nornicotine, mesmin, pseudobasine, anatine and cotinine;

[0056] Reagents: triethylamine, chloroform, 5% sodium hydroxide solution, methyl tert-butyl ether;

[0057] Instruments: gas chromatography mass spectrometry (Agilent); fused silica capillary column (30mx0.25mmx0.25μm); vortex oscillator, (Advancedmulti company) desktop high-speed refrigerated centrifuge (SIGMA company); AE163 electronic balance, sense: 0.0001g, (Mettler, Switzerland). Organic phase syringe filter, 13mm×0.22μm (Shanghai Anpu Scientific Instrument Co., Ltd.).

[0058] 2. Sample handling:

[0059] Weigh 1g of e-cigarette liquid sample into a 50mL centrifuge tube, then add 1.0~6.0mL of 10% sodium hydroxide solution and let stand for 10min. Take 10 mL of 0.01% triethylamine in methyl tert-butyl ether solution into a centrifuge tube, and add 200 μL of internal standard solution (the concentration of 2-methylqu...

example 2

[0073] The specific steps are as described in Example 1, another sample B is selected, and the measured content is shown in Table 5.

[0074] Table 5 Test results in e-cigarette liquid and e-cigarette mainstream smoke

[0075] compound E-liquid content (μg / g) Mainstream smoke content (μg / puff) Nicotine 10633 2.74 Nornicotine 62.872 0.15 Mesmin 76.522 0.06 Pseudine 55.518 0.72 neonicotinoid 13.165 0.13 Cotinine 20.695 0.23

example 3

[0077] The specific steps are as described in Example 1. Another sample C was selected, and the measured nicotine content is shown in Table 6.

[0078] Table 6 Test results in e-cigarette liquid and e-cigarette mainstream smoke

[0079] compound E-liquid content (μg / g) Mainstream smoke content (μg / puff) Nicotine 17084 33.99 Nornicotine 16.464 0.16 Mesmin 59.12 0.10 Pseudine 32.777 0.00 neonicotinoid 93.425 0.41 Cotinine 22.177 0.00

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Abstract

The invention belongs to the technical field of physical and chemical inspection of nicotine liquid and main stream smoke of an electronic cigarette, in particular to a method for detecting nicotine and secondary alkaloids (nicotine, nornicotine, myosmine, anabasine, neonicotine and conitine) in nicotine liquid and main stream smoke of the electronic cigarette. The method comprises the steps of extracting nicotinic substances in nicotine liquid and main stream smoke of the electronic cigarette by utilizing a centrifugal tube containing 0.01 percent triethylamine / tert-butyl methyl ether solution, and analyzing the nicotinic substances according to a gas chromatography / mass-spectrum combined method (GC-MS). The method is fast, effective, simple in pretreatment, smaller than 6.5 percent in average relative standard deviation, and 87.1 to 94.8 percent in the average recovery rate of each index, and has the advantages of being fast, accurate, and high in sensitivity and repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical inspection of electronic cigarette liquid, and specifically relates to the detection of nicotine, nornicotine, mesmin, anatine, anonicotine and cotinine in electronic cigarette liquid and mainstream smoke of electronic cigarette Determination technique. Background technique [0002] Tobacco alkaloids are an extremely important class of chemical components in tobacco. Nicotine, nornicotine, anatabine, and anatabine are the main alkaloids, and other alkaloids except nicotine are usually called "secondary alkaloids". Nicotiana belongs to the family Solanaceae Nicotiana, which can be divided into 3 subgenera, namely common tobacco, Nicotiana nicotiana and Pidonga, a total of 66 species in 14 groups. The alkaloid content varies greatly. Among them, 50-60% of the species use nicotine as the main alkaloid, and nicotine accounts for more than 80% of the total alkaloids, including common t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/025G01N2030/062
Inventor 张洪非庞永强李雪朱风鹏姜兴益罗彦波李翔宇刘洋
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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