Hybrid larch micro-cuttage seedling growing method

A technology for larch and micro-cutting, which is applied in the field of forestry genetics and breeding, can solve the problem that the number of cuttings and seedlings cannot meet the requirements of larch, and achieves the effects of a large number of twigs, fast germination, convenient intensive management and factory production.

Active Publication Date: 2016-03-02
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, larch cuttings need to use the semi-lignified twigs of the year as cuttings. The current technical application in production can only be used for one-time outdoor cuttings from late June to early July every year, depending on the age, location, season and number of materials. Due to the limitation of various factors, the number of seedlings raised by cuttings cannot meet the market demand for improved seedlings of larch

Method used

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  • Hybrid larch micro-cuttage seedling growing method
  • Hybrid larch micro-cuttage seedling growing method
  • Hybrid larch micro-cuttage seedling growing method

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Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1, the screening of hybrid larch micro-cuttage best condition for raising seedlings

[0045] Data statistics and analysis methods:

[0046] Plant sterile survival rate = number of sterile plants / number of inoculated plants. The average number of cryptobuds germinated from a single twig = the number of germinated cryptobuds / the number of inoculated twigs. Branching rate of cryptobuds = number of cryptobuds into branches / total number of cryptobuds inoculated. Adventitious bud induction rate = number of dormant buds successfully induced adventitious buds / number of dormant buds inoculated. The average number of adventitious buds formed by a single germinated dormant bud = the total number of adventitious buds / the number of successfully induced dormant buds. Rooting rate = number of rooted shoots / total number of shoots inoculated by treatment. The average number of roots = the total number of roots in the treatment / the number of rooted shoots in the treatmen...

Embodiment 2

[0073] Embodiment 2, hybrid larch micro-cutting seedling raising method

[0074] 1. Construction of miniature ear picking nursery

[0075] The biennial plants of the Riyong 8×Chang 18-10 hybrid clone bred by the Forestry Institute of the Chinese Academy of Forestry with excellent growth traits and cutting rooting ability were cultivated in a climate chamber as hybrid larch miniature plants. The mother plant of the picking nursery. The climate chamber uses incandescent lamps as the light source, the light period is 16 hours a day, the light intensity is 2000-3000lx, the temperature is 23±2°C, and the air humidity is 50%.

[0076] 2. Preparation of rooting medium

[0077] Improved L 9 Components of the medium and their concentrations, macroelements: KNO 3 633.33mg / L, NH 4 NO 3 550mg / L, KH 2 PO 4 56.67mg / L, CaCl 2 2H 2 O110.67mg / L, MgSO 4 ·7H 2 O60.18mg / L; Iron salt: FeNaEDTA36.70mg / L; Trace elements: H 3 BO 3 6.20mg / L, ZnSO 4 ·H 2 O8.60mg / L, MnSO 4 ·H 2 O16.90m...

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Abstract

The invention relates to a hybrid larch micro-cuttage seedling growing method. The method comprises the following steps: (1) preparing cutting materials, including acquiring current-year twigs from Japanese larch*Larix olgensis hybridal clone plants, disinfecting; (2) inducing rooting, being performed by a two-step rooting method, that is, after rooting induction of the disinfected twigs in an improved L9 culture medium containing auxin, transferring the twigs in an improved L9 culture medium which does not contain auxin to culture for rooting, the whole process being carried out in an illumination incubator. Using the method to perform cutting obviously improves cutting rooting ratio of hybrid larch, twig rooting percentage reaches up to more than 85.0%, and adventitious roots can be seen after about 30 days. The average number of rooting roots is 11. The method effectively shortens seedling growing period of good planting materials, enlarges propagation coefficient, and opens up a new approach for culturing hybrid larch excellent seedlings.

Description

technical field [0001] The invention relates to a hybrid larch seedling raising method by micro-cuttage, which belongs to the field of forestry genetic breeding. Background technique [0002] Larix is ​​a deciduous tree of the Pinaceae family. It is mainly distributed in the forest areas of Northeast my country, Inner Mongolia, and alpine areas in North China and Southwest China. It is an important fast-growing timber tree species and ecological afforestation tree species in my country. Less advantages. Due to the combination of the fast growth of the female parent of Japanese larix and the environmental adaptability of the male parent of Larix longifolia, the day×long hybrid larch showed obvious super-parent heterosis in terms of growth and resistance. However, due to factors such as the lack of inter-species flowering and the lack of seeds, the artificial preparation of hybrid seeds cannot meet the production needs. [0003] Somatic embryogenesis and budding of larch in N...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C05G1/00
CPCA01G31/00C05B7/00C05C1/00C05C5/02C05C11/00C05D1/00C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00
Inventor 张守攻孙晓梅李魁鹏韩华
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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