Detection method of high-temperature-resistant aerogenesis acidophil in table vinegar

Abstract

The invention provides a detection method of high-temperature-resistant aerogenesis acidophil in table vinegar. The detection method specifically comprises the following steps: (1) diluting and uniformly shaking a to-be-detected sample; (2) continuously performing gradient dilution on a diluted solution and applying a diluent sample to an experiment on microbiology; (3) performing static cultivation on a flora, dyeing and observing by using a microscope to determine the type of contamination bacteria, and finally detecting physicochemical indexes of the contamination; the detection method can detect a strain which generates an aerogenesis acidophilic phenomenon during table vinegar brewing through a simplest and effective way and is applicable to the detection on yeast, raw material and adjunct for brewing table vinegar as well as a bottle and a cover required by canning sealing, and is beneficial for the judgment on pollution sources and pollution condition, and is suitable for the requirement of large-scale industrial production; the pollution range is decreased and the loss of enterprises is reduced.

Description

technical field [0001] The invention relates to the technical field of detection of brewed vinegar, in particular to a detection method for high-temperature-resistant gas-producing acidophilus bacteria in vinegar. Background technique [0002] Vinegar is made from grains through the combination of microbial koji making, saccharification, alcoholic fermentation, acetic acid fermentation and various amino acids and enzyme preparations. In the production process of finished vinegar, there will be microorganisms, such as: mold, yeast, a certain number of bacteria and actinomycetes. According to the fact that vinegar belongs to the genus of seasoning food and integrates its flavor, the sterilization conditions are: keep the temperature at 60-80°C for 20-30 minutes or heat the vinegar solution to 100°C for 5 minutes. If there is no special pollution, the above conditions can sterilize the vinegar The bacteria in the brewing process are killed, and it meets the microbial requireme...

AI Technical Summary

Problems solved by technology

Common microbial detection methods include: culture membrane method, spiral plate counting method, filter membrane method, ATP bioluminescence method, electrical impedance method, color change method, flow cytometry technology and laser scanning technology, etc. These technologies are expensive and not suitable for Microbiological testing by industrial production companies