Serratia nematodiphila strain and application thereof
A technology of Serratia nematophila and nematophila, applied in the application field of Serratia nematophila and its growth-promoting characteristics, can solve problems such as poor crop rotation conditions, deterioration of soil physical properties, and nutritional imbalance
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[0025] The present invention will be further described below in conjunction with embodiment.
[0026] Serratianematodiphila PR4 of the present invention, deposit unit: General Microbiology Center (CGMCC), China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, deposit date: 2014 On August 28, the deposit number: CGMCCNo.9614.
[0027] (1) Screening of CGMCCNo.9614
[0028] Enrichment process in beef extract peptone liquid medium: Take tobacco rhizosphere soil collected from Hunan Sangzhi Guandiping and Liuyang Tobacco Institute, a total of 10 samples, numbered G1, G2, G3, G4, G5, L1 , L2, L3, L4, L5, each soil sample was weighed 10g, added to 100mL beef extract peptone liquid medium, 191r / min, 37°C shaker shaking culture for 24h.
[0029] The process of degrading protein with skimmed milk powder as the substrate: after taking the enriched culture solution and serially diluting it, from 10 -1 、10 -2 ...
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