Method for mild and massive preparation of mineralization silk fibroin films

A silk fibroin protein, a large number of technologies, applied in medical science, prosthesis and other directions, can solve problems such as harsh preparation conditions, and achieve the effects of simple preparation process, controllable morphology, and mild preparation conditions

Active Publication Date: 2016-02-17
杭州千岛湖蓝色天使实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The formation of a large number of hydroxyapatite nanorods was only observed in the patent with application number 201510406788, but the preparation conditions are harsh and need to be carried out at 100 °C

Method used

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  • Method for mild and massive preparation of mineralization silk fibroin films
  • Method for mild and massive preparation of mineralization silk fibroin films

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of silk fibroin film: a silk fibroin aqueous solution with a mass fraction of 5% was cast at 37°C to form a film;

[0019] (2) Enzyme solution pretreatment of silk fibroin film: Soak the silk fibroin film in protease XIV solution with a concentration of 1U / mL for 24 hours, place it in a shaker at 37°C, and control the speed at 100 rpm. Then take out and wash with phosphate buffer solution (0.01mol / L PBS), and dry at room temperature;

[0020] (3) Phosphate deposition: Soak the silk fibroin film pretreated by protease in PBS solution with a phosphate ion concentration of 0.1mol / L, then place it at 37°C for static incubation, take it out after 3 days, wash it with distilled water, and keep it at room temperature Dried to obtain a phosphate-mineralized silk fibroin film.

[0021] (4) Calcium salt deposition: immerse the phosphate-mineralized silk fibroin film in a calcium chloride or calcium nitrate solution with a concentration of 0.2 mol / L for 24 hours t...

Embodiment 2

[0023] (1) Preparation of silk fibroin film: a silk fibroin aqueous solution with a mass fraction of 15% was cast at 60°C to form a film;

[0024] (2) Enzyme solution pretreatment of silk fibroin film: Soak the silk fibroin film in protease XIV solution with a concentration of 2U / mL for 24 hours, place it in a shaker at 37°C, and control the speed at 100 rpm. Then take out and wash with phosphate buffer solution (0.01mol / L PBS), and dry at room temperature;

[0025] (3) Phosphate deposition: Soak the silk fibroin film pretreated by protease in PBS solution with a phosphate ion concentration of 0.1mol / L, then place it at 37°C for static incubation, take it out after 3 days, wash it with distilled water, and keep it at room temperature Dried to obtain a phosphate-mineralized silk fibroin film.

[0026] (4) Calcium salt deposition: immerse the phosphate-mineralized silk fibroin film in a calcium chloride or calcium nitrate solution with a concentration of 0.2 mol / L for 24 hours ...

Embodiment 3

[0028] (1) Preparation of silk fibroin film: a silk fibroin aqueous solution with a mass fraction of 30% was cast at 37°C to form a film;

[0029] (2) Enzyme solution pretreatment of silk fibroin film: Soak the silk fibroin film in protease XIV solution with a concentration of 6U / mL for 2 hours, place it in a shaker at 37°C, and control the speed at 200 rpm. Then take out and wash with phosphate buffer solution (0.01mol / L PBS), and dry at room temperature;

[0030] (3) Phosphate deposition: Soak the silk fibroin film pretreated by protease in PBS solution with a phosphate ion concentration of 0.05mol / L, then place it at 37°C for static incubation, take it out after 3 days, wash it with distilled water, and keep it at room temperature Dried to obtain a phosphate-mineralized silk fibroin film.

[0031] (4) Calcium salt deposition: immerse the phosphate-mineralized silk fibroin film in a calcium chloride or calcium nitrate solution with a concentration of 0.2 mol / L for 24 hours ...

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Abstract

The invention provides a method for mild and massive preparation of mineralization silk fibroin films. The method comprises the steps of silk fibroin film preparation, silk fibroin film enzyme solution preprocessing, phosphate deposition and calcium deposition so that the silk fibroin films with the completely-mineralized surfaces can be obtained. The obtained mineralization silk fibroin films can be used for bone repair materials for cartilage repairing, periosteum repairing, ligament repairing and the like. According to the method, no repeated soaking is needed, the preparation process is simple, the preparation conditions are mild, and the most preparation processes are performed at the temperature of 37 DEG C; the obtained mineralization area is large, the quantity is large, and the shape is controllable.

Description

technical field [0001] The invention relates to a preparation method of a mineralized silk fibroin film, in particular to a mild and large-scale preparation method of a mineralized silk fibroin film. The method belongs to the field of biomaterials, and the prepared materials can be widely used in bone tissue repair. Background technique [0002] Due to its good biocompatibility, processability and mechanical properties, silk fibroin materials have attracted much attention in the field of biomaterials, especially in bone repair materials. The important performance of bone repair materials is that they can achieve osseointegration with bone tissue after implantation, because bone tissue is rich in organic substance collagen and inorganic substance hydroxyapatite. A single silk fibroin material is slow to calcify in the body. In order to improve this characteristic, the mineralization of silk fibroin material has received a lot of attention. [0003] The patent application nu...

Claims

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Application Information

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IPC IPC(8): C08J7/14C08J5/18C08L89/00C08L1/02C08L5/00C08L101/00C08L5/08A61L27/22A61L27/54
Inventor 何丹农周涓金林宇阳俊朱君
Owner 杭州千岛湖蓝色天使实业有限公司
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