Mold-culture-containing enzyme special for anthony pigs and preparation method thereof
A technology for mold cultivation and suckling pigs, which is applied in the field of special enzymes for suckling pigs and their preparation, can solve the problems that the function of the culture and the preparation method need to be improved, the activity of the enzyme preparation needs to be further improved, the special enzyme enzyme system is incomplete, etc. Intestinal flora balance, growth and reproduction inhibition, animal growth promotion effect
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Embodiment 1
[0060] Example 1 Preparation of raw materials
[0061] Preparation of mold culture:
[0062] The preparation method of the mold culture includes the following steps:
[0063] (1) Strain activation
[0064] The well-preserved slant strain of Aspergillus usamii CCTCCNO: M2013601 was inoculated into the slant medium, cultured at 32°C for 40 hours to activate the strain, and so activated 3 times;
[0065] (2) Expanded cultivation of liquid seeds
[0066] ①First-level seed culture: Wash the spores of the activated slant strain with sterile water in step (1) and put it into a 500ml shake flask. The liquid seed culture medium is filled with 100ml and cultivated on a shaker at 32°C and 100rpm for 40h;
[0067] ②Secondary seed culture: Put the first-level seed into a 500ml second-level seed shaker at 10% of the inoculum, and the cultivation conditions are the same as those of the first-level seed;
[0068] ③Three-level seed culture: Put the second-level seeds into a 5000 ml third-level seed shaker ...
Embodiment 2
[0085] A special enzyme for suckling piglets containing mold culture, consisting of the following parts by weight:
[0086] 42 parts of mold culture, 40 parts of plant extracts, 35 parts of amylase, 35 parts of acid xylanase, 25 parts of cellulase, 25 parts of β-glucanase, 18 parts of pectinase, 12 parts of mannase Parts, 12 parts of phytase, 8 parts of Chinese herbal medicine extract, 7 parts of protective agent, 7 parts of activator, 4 parts of antioxidant;
[0087] The mold cultures, plant extracts, and Chinese herbal medicine extracts were all prepared in Example 1;
[0088] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0089] The protective agent is composed of the following parts by weight of raw materials: 25 parts of trehalose, 25 parts of NaCl, (NH 4 ) 2 SO 4 12 parts, 12 parts cysteine;
[0090] The activator is uniformly mixed with inorganic salts of the following mass components: 35 parts of zin...
Embodiment 3
[0095] A special enzyme for suckling piglets containing mold culture, consisting of the following parts by weight:
[0096] 35 parts of mold culture, 35 parts of plant extract, 30 parts of amylase, 30 parts of acid xylanase, 20 parts of cellulase, 20 parts of β-glucanase, 15 parts of pectinase, 10 parts of mannase Parts, 10 parts of phytase, 6 parts of Chinese herbal medicine extract, 5 parts of protective agent, 5 parts of activator, 3 parts of antioxidant;
[0097] The mold cultures, plant extracts, and Chinese herbal medicine extracts were all prepared in Example 1;
[0098] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0099] The protective agent is composed of the following parts by weight of raw materials: 20 parts of trehalose, 20 parts of NaCl, (NH 4 ) 2 SO 4 10 parts, 10 parts cysteine;
[0100] The activator is uniformly mixed with inorganic salts of the following mass components: 30 parts of zinc...
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