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Mold-culture-containing enzyme special for anthony pigs and preparation method thereof

A technology for mold cultivation and suckling pigs, which is applied in the field of special enzymes for suckling pigs and their preparation, can solve the problems that the function of the culture and the preparation method need to be improved, the activity of the enzyme preparation needs to be further improved, the special enzyme enzyme system is incomplete, etc. Intestinal flora balance, growth and reproduction inhibition, animal growth promotion effect

Active Publication Date: 2016-02-10
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Chinese patent CN103875935A discloses a special enzyme for suckling piglets containing probiotics and a preparation method thereof. The special enzyme for suckling piglets contains a culture of Bacillus licheniformis; Enzymes and preparation methods thereof, the special enzyme for suckling piglets contains Aspergillus niger culture; the above-mentioned published patent scientifically compounded microbial cultures containing different types of enzyme preparations on the basis of feed compound enzymes, which can improve the health of suckling piglets. Immune function and production performance have a certain effect, but the activity of the enzyme preparation in the culture needs to be continuously improved, the function and preparation method of the culture need to be improved, the special enzyme enzyme system is incomplete (excluding plant enzymes), and the special enzyme components have no freedom to add and eliminate Antioxidant that bases and enhances enzyme activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation of raw materials

[0061] Preparation of mold culture:

[0062] The preparation method of the mold culture includes the following steps:

[0063] (1) Strain activation

[0064] The well-preserved slant strain of Aspergillus usamii CCTCCNO: M2013601 was inoculated into the slant medium, cultured at 32°C for 40 hours to activate the strain, and so activated 3 times;

[0065] (2) Expanded cultivation of liquid seeds

[0066] ①First-level seed culture: Wash the spores of the activated slant strain with sterile water in step (1) and put it into a 500ml shake flask. The liquid seed culture medium is filled with 100ml and cultivated on a shaker at 32°C and 100rpm for 40h;

[0067] ②Secondary seed culture: Put the first-level seed into a 500ml second-level seed shaker at 10% of the inoculum, and the cultivation conditions are the same as those of the first-level seed;

[0068] ③Three-level seed culture: Put the second-level seeds into a 5000 ml third-level seed shaker ...

Embodiment 2

[0085] A special enzyme for suckling piglets containing mold culture, consisting of the following parts by weight:

[0086] 42 parts of mold culture, 40 parts of plant extracts, 35 parts of amylase, 35 parts of acid xylanase, 25 parts of cellulase, 25 parts of β-glucanase, 18 parts of pectinase, 12 parts of mannase Parts, 12 parts of phytase, 8 parts of Chinese herbal medicine extract, 7 parts of protective agent, 7 parts of activator, 4 parts of antioxidant;

[0087] The mold cultures, plant extracts, and Chinese herbal medicine extracts were all prepared in Example 1;

[0088] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;

[0089] The protective agent is composed of the following parts by weight of raw materials: 25 parts of trehalose, 25 parts of NaCl, (NH 4 ) 2 SO 4 12 parts, 12 parts cysteine;

[0090] The activator is uniformly mixed with inorganic salts of the following mass components: 35 parts of zin...

Embodiment 3

[0095] A special enzyme for suckling piglets containing mold culture, consisting of the following parts by weight:

[0096] 35 parts of mold culture, 35 parts of plant extract, 30 parts of amylase, 30 parts of acid xylanase, 20 parts of cellulase, 20 parts of β-glucanase, 15 parts of pectinase, 10 parts of mannase Parts, 10 parts of phytase, 6 parts of Chinese herbal medicine extract, 5 parts of protective agent, 5 parts of activator, 3 parts of antioxidant;

[0097] The mold cultures, plant extracts, and Chinese herbal medicine extracts were all prepared in Example 1;

[0098] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;

[0099] The protective agent is composed of the following parts by weight of raw materials: 20 parts of trehalose, 20 parts of NaCl, (NH 4 ) 2 SO 4 10 parts, 10 parts cysteine;

[0100] The activator is uniformly mixed with inorganic salts of the following mass components: 30 parts of zinc...

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PUM

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Abstract

The invention discloses mold-culture-containing enzyme special for anthony pigs and a preparation method thereof and belongs to the technical field of preparation of enzyme preparations. Barley malt, burnt malt, figs and pineapples which are rich in a variety of enzyme systems and garlic, vanilla beans, illicium verum and fennel which can enhance animal appetite and resist to and inhibit bacteria serve as main raw materials, the low temperature wall breaking and extraction technologies are adopted, effective constituents in the raw materials are extracted to the greatest extent, natural plant extract is prepared, meanwhile, aspergillus usamii which can produce high-vitality acid protease serves as an original strain for producing a mold culture containing high-vitality acidic protease, Chinese herbal extract, protective agents, activating agents, anti-oxidant and other food-grade feed enzyme are compounded scientifically, by means of the obtained feed compound enzyme, safe and comprehensive digestive enzyme is provided for the anthony pigs, in addition, pleasant fragrance can be brought to feed, appetite of the anthony pigs is enhanced, the digestion and absorption functions of the stomachs and intestines of the anthony pigs are effectively improved, and productivity of the anthony pigs is raised.

Description

Technical field [0001] The invention belongs to the technical field of enzyme preparation preparation, in particular to a special enzyme for suckling piglets containing mold culture and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations in feed: 1. Degrade anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, causing indigestion and decreased production performance. 2. Improve the utilization of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or exist in certain structural forms that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus exists in the form of phytate phosphorus). 3. Degrade some specific chemical bonds in the raw materials. These chemical bonds cannot be degraded by the animal's own enzy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/16A23K1/18
Inventor 李洪兵李海清张锦杰朱永明胡永明易敏
Owner 湖南新鸿鹰生物工程有限公司
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