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Microfluidic chip, detection system and device used for rapid DNA detection

A microfluidic chip and detection device technology, applied in the field of DNA rapid detection systems and devices, can solve the problems of unsuitable genetic research centers and clinical diagnosis laboratories, large consumption of DNA analysis samples, and low analysis throughput, etc. The effect of rapid PCR amplification and real-time fluorescence detection, improving reliability and repeatability, and increasing sample throughput

Inactive Publication Date: 2016-02-03
融智生物科技(青岛)有限公司 +1
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a microfluidic chip for rapid DNA detection, and a DNA rapid detection system and device including the chip, so as to solve the problem of large consumption of DNA analysis samples and long operation period in the prior art. , cumbersome steps, low precision, etc., and solve the existing microfluidic chip and DNA detection system with insufficient automation and low analysis throughput, which is not suitable for the needs of biological laboratories, genetic research centers and clinical diagnostic laboratories. question

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[0050] In order to illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments and accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.

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Abstract

The present invention discloses a microfluidic chip for rapid DNA detection, and the chip includes a liquid storage unit, a nucleic acid extraction unit, an amplification and detection unit and a fluid control unit. The present invention further discloses a system and a device comprising the microfluidic chip for rapid DNA detection. The microfluidic chip can achieve repeated and automated nucleic acid extraction and fast and efficient PCR amplification and real-time fluorescence detection. At the same time, by use of a disc array structure, a plurality of samples can be analyzed in parallel, so that the method is expected to be used in scientific research and clinical body fluid sample DNA rapid detection.

Description

technical field [0001] The invention relates to biomedical instruments, in particular to a microfluidic chip for rapid DNA detection, and a DNA rapid detection system and device comprising the chip. Background technique [0002] The analysis of DNA in body fluids (such as blood, urine, etc.), especially the detection of gene markers and gene variation detection is an important part of biomedical research, and it is also an important way for genetic diagnosis and genetics research. At present, the methods used for body fluid DNA analysis mainly involve sample DNA extraction, target gene PCR amplification, electrophoretic separation and detection, or real-time fluorescent PCR detection. Generally, conventional instruments and equipment are used to complete different operations. In recent years, There are also nucleic acid analysis methods and instruments using microfluidic technology, mainly the miniaturization and scale integration of routine operations (such as chip nucleic ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34
CPCC12Q1/686C12Q2563/107
Inventor 钟润涛李运涛周晓光施建春
Owner 融智生物科技(青岛)有限公司
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