A kind of method of artificial inoculation of spodoptera spp.
A technology of two-point moth and artificial inoculation, which is applied in hunting equipment, application, animal husbandry, etc., can solve the problems of test consistency and accuracy difficult to control, and achieve strong activity, high chance of feeding, and test consistent effect
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Embodiment 1
[0035] Example 1: Screening test for ages of Spodoptera spp. and corn seedlings suitable for inoculation.
[0036] (1), test material: in this laboratory according to routine method, the same age, healthy and lively 2nd instar, 2nd instar, 3rd instar, 4th instar and 5th instar larvae were raised. The corn variety is Zhengdan 958.
[0037](2), test treatment: plant corn in a large indoor pot or set up a gauze grid protection area (or a large pot in a greenhouse), each treatment area is 1 square meter, the planting density is 8 plants / square meter, and the soil surface is artificially laid with a layer thickness of 1.5cm of wheat straw, in order to facilitate the larvae habitat, respectively in the 1-leaf-1-heart stage, 2-leaf-1-heart stage, 3-leaf stage, and 4-leaf stage of corn, each treatment was respectively inserted into the 2nd, 3rd, 4th or 5th instar The number of larvae and inoculated insects was divided into 5, 10, and 20 heads / treatment, and repeated 3 times. 2-7 day...
Embodiment 2
[0047] Embodiment 2: Anti-escape device and feeding damage test
[0048] (1) Test materials: healthy and lively 3rd instar larvae of Spodoptera spp. in the same age group raised according to conventional methods in this laboratory. The corn variety is Zhengdan 958.
[0049] (2) Test treatment: height 10cm, Zhengdan 958 sown in a single pot of a plastic flowerpot with a diameter of 15cm, the soil depth in the pot is 2-3cm apart from the upper edge of the flowerpot, and 3 seeds of Zhengdan 958 are sown in each pot; the seedlings grow to 2 At the -3-leaf stage, select 2-3 leaf-stage corn seedlings with uniform growth, keep 1 seedling in each pot, and pull out the rest; water 1 day before inoculation to ensure that the soil has a certain humidity. Before inoculation, the inoculation environment was treated, respectively (a) direct inoculation, (b) covering the base of the stem with wheat straw and chaff, (c) covering the base of the stem with kraft confetti (see figure 1 ), (d)...
Embodiment 3
[0055] Embodiment 3: Utilize the method of the present invention to the in vivo determination comparative test of the insecticide of preventing and treating the larvae of Spodoptera spp.
[0056] (1) Test materials: healthy and lively 3rd instar larvae of Spodoptera spp. in the same age group raised according to conventional methods in this laboratory. The corn variety is Zhengdan 958. The medicaments were 20% thiamethoxam+20% cyantraniliprole seed coating (purchased from Syngenta); 60% imidacloprid suspension seed coating (purchased from Bayer Crop Science Co., Ltd.); Seed coating agent, carbosulfan poison bait and carbosulfan granules (carbosulfan test agents were purchased from Beinong (Haili) Zhuozhou Seed Coating Agent Co., Ltd.).
[0057] (2) Test treatment
[0058] Treatment 1: CK control (without any treatment);
[0059] Treatment 2: 20% thiamethoxam + 20% cyantraniliprole FS coated seeds (drug species ratio 1:333);
[0060] Treatment 3: 20% thiamethoxam + 20% cyan...
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