A kind of method of artificial inoculation of spodoptera spp.

A technology of two-point moth and artificial inoculation, which is applied in hunting equipment, application, animal husbandry, etc., can solve the problems of test consistency and accuracy difficult to control, and achieve strong activity, high chance of feeding, and test consistent effect

Inactive Publication Date: 2017-12-08
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at problems such as the escape of artificial inoculated larvae and the failure to bite the target host corn seedlings in the bioassay of insecticides used to prevent and control Spodoptera spp., the consistency and accuracy of the test are difficult to control, etc. The artificial inoculation method of Spodoptera moth; this method simulates the damage symptoms of corn seedlings bitten by the insect under natural conditions in the field under the condition of inoculating a single larva, so as to provide an effective method for the bioassay and screening of insecticides. way

Method used

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  • A kind of method of artificial inoculation of spodoptera spp.
  • A kind of method of artificial inoculation of spodoptera spp.
  • A kind of method of artificial inoculation of spodoptera spp.

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1: Screening test for ages of Spodoptera spp. and corn seedlings suitable for inoculation.

[0036] (1), test material: in this laboratory according to routine method, the same age, healthy and lively 2nd instar, 2nd instar, 3rd instar, 4th instar and 5th instar larvae were raised. The corn variety is Zhengdan 958.

[0037](2), test treatment: plant corn in a large indoor pot or set up a gauze grid protection area (or a large pot in a greenhouse), each treatment area is 1 square meter, the planting density is 8 plants / square meter, and the soil surface is artificially laid with a layer thickness of 1.5cm of wheat straw, in order to facilitate the larvae habitat, respectively in the 1-leaf-1-heart stage, 2-leaf-1-heart stage, 3-leaf stage, and 4-leaf stage of corn, each treatment was respectively inserted into the 2nd, 3rd, 4th or 5th instar The number of larvae and inoculated insects was divided into 5, 10, and 20 heads / treatment, and repeated 3 times. 2-7 day...

Embodiment 2

[0047] Embodiment 2: Anti-escape device and feeding damage test

[0048] (1) Test materials: healthy and lively 3rd instar larvae of Spodoptera spp. in the same age group raised according to conventional methods in this laboratory. The corn variety is Zhengdan 958.

[0049] (2) Test treatment: height 10cm, Zhengdan 958 sown in a single pot of a plastic flowerpot with a diameter of 15cm, the soil depth in the pot is 2-3cm apart from the upper edge of the flowerpot, and 3 seeds of Zhengdan 958 are sown in each pot; the seedlings grow to 2 At the -3-leaf stage, select 2-3 leaf-stage corn seedlings with uniform growth, keep 1 seedling in each pot, and pull out the rest; water 1 day before inoculation to ensure that the soil has a certain humidity. Before inoculation, the inoculation environment was treated, respectively (a) direct inoculation, (b) covering the base of the stem with wheat straw and chaff, (c) covering the base of the stem with kraft confetti (see figure 1 ), (d)...

Embodiment 3

[0055] Embodiment 3: Utilize the method of the present invention to the in vivo determination comparative test of the insecticide of preventing and treating the larvae of Spodoptera spp.

[0056] (1) Test materials: healthy and lively 3rd instar larvae of Spodoptera spp. in the same age group raised according to conventional methods in this laboratory. The corn variety is Zhengdan 958. The medicaments were 20% thiamethoxam+20% cyantraniliprole seed coating (purchased from Syngenta); 60% imidacloprid suspension seed coating (purchased from Bayer Crop Science Co., Ltd.); Seed coating agent, carbosulfan poison bait and carbosulfan granules (carbosulfan test agents were purchased from Beinong (Haili) Zhuozhou Seed Coating Agent Co., Ltd.).

[0057] (2) Test treatment

[0058] Treatment 1: CK control (without any treatment);

[0059] Treatment 2: 20% thiamethoxam + 20% cyantraniliprole FS coated seeds (drug species ratio 1:333);

[0060] Treatment 3: 20% thiamethoxam + 20% cyan...

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Abstract

The invention belongs to the field of pesticide bioassays, and specifically discloses an artificial inoculation method for the spodoptera moth. The present invention coats corn seeds with the medicament to be tested, or sprinkles poisonous baits or granules of the medicament to be tested at the stem base of corn seedlings; Inoculation, and the inoculation medium was placed at the base of the corn seedling stem, covered with a protective cover, and the larvae were placed on the inoculation medium for investigation. The present invention provides accurate, reliable method for the insecticide screening of preventing and treating the two-spotted moth; secondly, the 3rd instar larva that the present invention selects is strong to the sensitivity of medicament, food intake is big, and activity ability is strong, and its to corn seedling The feeding probability is large; in addition, the setting of the inoculation medium and the protective cover in the inventive method makes the larva escape rate low, and can avoid the predation of natural enemies; the 2-3 leaf stage corn seedling stem base tissue young Tender, more suitable for larvae to feed on, and the symptoms will be obvious after being bitten.

Description

technical field [0001] The invention belongs to the field of pesticide bioassays, and in particular relates to an artificial inoculation method for the spodoptera moth. Background technique [0002] Proxenus lepigone (Moschler), synonym: Athetis lepigone, belongs to the genus Athetis lepigone of the Science and Technology Committee of the Noctuid moth. In recent years, after the transformation of farming systems such as no-tillage or stubble sowing after wheat harvest, my country's Huanghuaihai summer corn plantation important new pests in maize seedling stage. Spodoptera moth mainly breaks out damage in wheat-corn inter-sowing cornfields. Its main pest generation larvae like dark, dark, and humid environments, and often hide under the broken wheat straw around corn seedlings to inhabit, feed, and move. The area with a lot of wheat stubble and wheat straw left over from the field has a large number of insect populations. [0003] The larvae of the two-spotted moth have comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01M99/00
Inventor 张海剑石洁郭宁宋健杨硕陈丹
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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