Method for efficiently removing epibiotic microorganisms on surfaces of large seaweed

Abstract

The invention belongs to the field of biotechnologies of seaweed, and relates to a method for efficiently removing epibiotic microorganisms on the surfaces of large seaweed. The method particularly includes removing the epibiotic microorganisms on the surfaces of the to-be-treated large seaweed under the resonance actions of quartz sand and the to-be-treated large seaweed. The method has the advantages that the epibiotic microorganisms on the surfaces of the large seaweed can be efficiently and thoroughly removed by the aid of the method, and the method is free of interference on endophytic microorganisms; the method can be used for separating and purifying endosymbiotic microorganisms of the seaweed and studying active natural products of the seaweed.

Description

technical field [0001] The invention belongs to the technical field of seaweed biology and relates to a method for efficiently removing epiphytic microorganisms on the surface of large seaweeds. Background technique [0002] Macroalgae belong to lower plants, mainly including brown algae, red algae and green algae. Many of these economic species are the objects of artificial cultivation, such as seaweed (Saccharinas pp.), wakame (Undaria pinnatifida), hijiki (Sargassumfusiforme) of brown algae, seaweed (Pyropias pp.) of red algae, red cabbage (Bangias pp.), Gracilaria spp., Gelidium spp., Eucheumas pp., and green algae Enteromorpha and Ulvas pp. [0003] Large seaweed can not only be used as healthy food (such as seaweed, red cabbage, kelp, wakame, enteromorpha), but also be used to extract and prepare important chemical raw materials such as iodine, mannitol, and alginate. In addition, recent studies have found that macroalgae are also important hosts of marine microorgan...

AI Technical Summary

Problems solved by technology

Since the epiphytic microorganisms of seaweed do not simply attach to the cell surface, but establish a relatively close connection with the host cell through a macromolecular network structure such as polysaccharides, proteins, and nucleic acids, it is difficult to effectively remove them with conventional mild methods and will Periphytic microorganisms

For example, physical methods such as co-oscillation of glass beads and algae, ultrasonic treatment, etc. have little effect on endophytic microorganisms, but studies have shown that they cannot completely remove epiphytic microorganisms

On the contrary, since the algae are often relatively fragile, the use of violent methods will lead to cell rupture or the killing of endophytic microorganisms.

For example, bleaching powder treatment in chemical methods can remove epiphytic microorganisms, but it will have a serious impact on endosymbiotic microorganisms, or even completely kill them

However, enzymatic methods generally require high-temperature enzymatic treatment, which will also have a significant impact on the diversity composition of endosymbiotic microorganisms.

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