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Potassium dissolving microorganism and application thereof in crop planting

A technology of microorganisms and microbial preparations, applied in the field of microbiology, can solve the problems of unstable yield increase effect, ineffective potassium solution effect, weak strain survival ability, etc., to achieve good crop seedling breeding, good soil improvement effect, and yield increase effect stable effect

Active Publication Date: 2016-01-27
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some progress has been made in the research of biological potassium fertilizers, there are still problems such as unstable yield increase effect, weak survival ability of bacteria in soil, and ineffective potassium-dissolving effect, and the combination of potassium-dissolving bacteria and mineral potassium fertilizers has not yet been seen. Reports on the use of alternative chemical potassium fertilizers in crop cultivation

Method used

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  • Potassium dissolving microorganism and application thereof in crop planting
  • Potassium dissolving microorganism and application thereof in crop planting
  • Potassium dissolving microorganism and application thereof in crop planting

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Screening and identification of Bacillus glialis NGW1

[0031] A large number of strains were screened from the soil. Select a single colony through a screening plate, and use an atomic absorption instrument to detect potassium in the fermentation supernatant to obtain the NGW1 strain. The specific screening method is as follows: take the potassium-dissolving bacteria isolation plate as the screening condition, and initially isolate the bacteria from the soil by the dilution method. For colonies with smooth edges, translucent, shiny, viscous colloids, and larger diameters, continue to streak culture until a single colony is obtained; activate the single colony and insert it into the re-screening medium of potassium-dissolving bacteria for 7 days, and use an atomic absorption instrument Potassium content in the fermentation supernatant was detected to obtain high-efficiency potassium-decomposing bacteria NGW1.

[0032] After such figure 1According to the phylogeneti...

Embodiment 2

[0081] Embodiment 2 is basically the same as Embodiment 1, and the effect is close, the difference is:

[0082] One, the present embodiment makes the potassium-decomposing microbial preparation with the fermentation liquid of bacillus colloidus NGW1: the volume ratio of wheat bran=1:2, and concrete steps comprise:

[0083] Activation: Inoculate Bacillus colioids NGW1 in the preservation medium for activation, and culture it in a constant temperature incubator at 30°C for 20 hours. The composition of the above preservation medium is: 5% sucrose, 0.5% dipotassium hydrogen phosphate, 0.5% magnesium sulfate, 0.5% calcium carbonate, 1.5% agar, pH 7.5, sterilized at 115°C for 20 minutes before use.

[0084] Seed liquid preparation: Pick a single colony of activated Bacillus colioids NGW1 on the plate and inoculate it into the liquid seed medium, and culture it at 25°C for 10 hours to reach the cell concentration OD 600 When it reaches 0.5-0.6, the strain seed solution is obtained. ...

Embodiment 3

[0093] Embodiment 3 is basically the same as Embodiment 1, and the effect is close, the difference is:

[0094] In this embodiment, the potassium-dissolving microbial preparation is made with the fermentation broth of Bacillus colloidus NGW1: wheat bran=1:10 by volume, and the specific steps include:

[0095] Activation: Inoculate Bacillus colioids NGW1 into the preservation medium for activation, and culture it in a constant temperature incubator at 35°C for 15 hours. The composition of the above preservation medium is: 3% sucrose, 1% dipotassium hydrogen phosphate, 1% magnesium sulfate, 1% calcium carbonate, 1.8% agar, pH 7.5, sterilized at 115°C for 20 minutes before use.

[0096] Seed liquid preparation: Pick a single colony of activated Bacillus colioids NGW1 on the plate and inoculate it into the liquid seed medium, and culture it at 35°C for 9 hours to reach the cell concentration OD 600 When it reaches 0.5-0.6, the strain seed solution is obtained. The composition of...

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Abstract

The invention provides a Bacillus mucilaginosus, collected under CCTCC NO: M2015175 and named Bacillus mucilaginosus NGW1, and solves the problems that an existing biological potassium fertilizer has instable microorganism potassium-dissolving effect and growth promoting effect and is lack of colonizing capacity. The Bacillus mucilaginosus NGW1 is further fermented and is compounded with a wheat bran carrier capable of promoting Bacillus mucilaginosus NGW1 to play, to obtain a potassium-dissolving microbial agent, which is applied to crops to give stable yield-increasing effect, high ability of bacteria to survive in soil and good soil improvement effect. A fertilizer application method increasing crop yield is further provided, germs are proliferated using a carrier as a nutrient substance to further disintegrate minerals, thus promoting soil consolidation, potassium release and potassium absorption by crops, the method can decrease the usage of chemical potassium fertilizer, save resources, improve the soil, and increase crop yield and quality. A method of significantly increasing crop germination rate is also provided which is good for raising crop seedlings.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a potassium-decomposing microorganism with strong potassium-decomposing ability, high colonization ability, certain growth-promoting ability and harmless to the environment and its application in crop planting. Background technique [0002] Potassium is the third most needed by plants, and the content of potassium determines the growth and quality of plants. However, potassium content in crops in my country is still low. In the process of planting crops in my country, chemical potassium fertilizers have been used excessively for a long time to increase the potassium content. Although this approach increases the potassium content of crops in a short period of time, long-term large-scale and single application is likely to cause soil compaction, soil structure damage, and soil microbial diversity decline, which hinders crops from absorbing potassium. Potassium is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/02A01P21/00C12R1/07
Inventor 陈守文张健行徐迪红余君王瑞王昌军曾衡杨欢陈建刚马昕李进平杨勇
Owner HUBEI UNIV
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