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Hirudo small peptide effective part medicine for treating diabetes and complications thereof

An effective part and diabetes technology, which is applied to the effective part composition of leech small peptide, the effective part composition of traditional Chinese medicine and its preparation field, can solve the problems of low active ingredient content, large dosage, poor drug effect, etc., and achieve stable curative effect, The effect of small doses and clear ingredients

Inactive Publication Date: 2016-01-27
ANHUI BIOLOGICAL PEPTIDE IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current Chinese medicine compound recipes have low content of active ingredients, large doses, and poor efficacy.

Method used

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  • Hirudo small peptide effective part medicine for treating diabetes and complications thereof
  • Hirudo small peptide effective part medicine for treating diabetes and complications thereof
  • Hirudo small peptide effective part medicine for treating diabetes and complications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Prescription: leech small peptide 1.2kg, astragalus total saponins 0.4kg, wolfberry total polysaccharides 3kg, cornel glucosides 0.6kg

[0084] Preparation method: Preparation of small leech peptides: take leeches, crush them properly, add 10 times the amount of water to homogenate for 10 minutes, heat and boil for 10 minutes, let cool to room temperature, use hydrochloric acid to adjust the pH to 2.0±0.1, and then add 8% of the substrate Pepsin, hydrolyze at 37°C for 1 hour, then use sodium hydroxide solution to adjust the pH to 8, add trypsin with 8% substrate, and hydrolyze at 45°C for 3 hours, stir constantly and adjust the pH to 7~ Within the range of 10, after the enzymolysis is completed, continue to heat up and boil for 10 minutes, let it cool, centrifuge, collect the supernatant, adjust the pH to 6.5-7.0, add 2% activated carbon, stir evenly, filter, and the filtrate has a molecular weight cut-off of 3kD The hollow fiber membrane is used for ultrafiltration, th...

Embodiment 2

[0090] Prescription: Take 80g of small leech peptides, 20g of total astragalus saponins, 140g of total polysaccharides from wolfberry, and 40g of total glycosides from cornel

[0091] Preparation method: Preparation of small leech peptides: take leeches, crush them properly, add 15 times the amount of water to homogenate for 15 minutes, heat and boil for 30 minutes, let cool to room temperature, use hydrochloric acid to adjust the pH to 2.0±0.1, and then add 10% of the substrate Pepsin, hydrolyze at 37°C for 2 hours, then use sodium hydroxide solution to adjust the pH to 9, add trypsin with 10% substrate, and digest at 45°C for 4 hours, stir constantly and adjust the pH to 7~ Within the range of 10, after the enzymolysis is completed, continue to heat up and boil for 15 minutes, let it cool, centrifuge, collect the supernatant, adjust the pH to 6.5-7.0, add 3% activated carbon, stir evenly, filter, and the filtrate has a molecular weight cut-off of 3kD The hollow fiber membran...

Embodiment 3

[0097] Prescription: Take 300g of small leech peptides, 300g of total astragalus saponins, 1600g of total polysaccharides of wolfberry, and 400g of total glycosides of cornel.

[0098] Preparation method: Preparation of small leech peptides: Take leeches, crush them appropriately, add 5 times the amount of water to homogenate for 55 minutes, heat and boil for 5 minutes, let cool to room temperature, use hydrochloric acid to adjust the pH to 2.0±0.1, and then add 0.5% of the substrate Pepsin, hydrolyze at 30°C for 1 hour, then use sodium hydroxide solution to adjust the pH to 8, add trypsin with a substrate amount of 0.5%, enzymolyze at 45°C for 3 hours, stir constantly and adjust the pH to 7~ Within the range of 10, after the enzymolysis is completed, continue to heat up and boil for 5 minutes, let it cool, centrifuge, collect the supernatant, adjust the pH to 6.5-7.0, add 1% activated carbon, stir evenly, filter, and the filtrate has a molecular weight cut-off of 3kD The holl...

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Abstract

The invention discloses a hirudo small peptide effective part composition for treating diabetes and complications thereof, and belongs to the field of traditional Chinese medicines. The composition consists of hirudo small peptides extracted from hirudo, radix astragali total saponins extracted from radix astragali, fructus lycii total polysaccharides extracted from fructus lycii, and cornus officinalis total glycosides extracted from cornus officinalis according to a certain proportion, pharmaceutical conventional accessories are added to obtain various dosage forms, and the composition can be clinically used for preventing or treating diabetes and complications thereof.

Description

technical field [0001] The present invention relates to a leech small peptide effective part composition with the function of preventing or treating diabetes and its complications and a preparation method thereof, in particular the traditional Chinese medicine effective part composition extracted from leeches, astragalus, medlar and dogwood and its preparation method. The preparation method belongs to the field of traditional Chinese medicines. Background technique [0002] Diabetes mellitus (DM) is a lifelong endocrine disease caused by a variety of causes of glucose metabolism disorders. Currently, the number of DM patients in the world is about 240 million, and it is estimated that by 2025, the number will reach 350 million. The disorder of glucose metabolism caused by diabetes leads to microvascular disease, which in turn leads to a series of diabetic complications, including diabetic retinopathy, diabetic nephropathy and diabetic foot. Diabetic nephropathy (DN) and dia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/62A61P3/10A61K36/815
Inventor 刘国飞代龙卢宁
Owner ANHUI BIOLOGICAL PEPTIDE IND RES INST
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