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Extraction process of environment-friendly blue pigment

An extraction process and an environment-friendly technology, applied in the direction of azo dyes, organic dyes, chemical instruments and methods, etc., can solve the problems of difficult effective separation of protein and pigment, large consumption of organic solvents, and easy adhesion to bottle walls, etc., to achieve extraction The process is environmentally friendly, improves the quality of the pigment, and is conducive to the effect of preservation

Active Publication Date: 2017-11-24
HEZE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of blue pigment is generally separated by centrifugation and solid-liquid separation, and then alcohol precipitation, spray drying and other methods are used. It is often difficult to effectively separate protein and pigment, and the consumption of organic solvents such as ethanol is large, and the recovery cost is high; although spray drying is fast, it can High consumption, and because it contains protein, it is often easy to stick to the bottle wall and difficult to separate

Method used

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  • Extraction process of environment-friendly blue pigment
  • Extraction process of environment-friendly blue pigment
  • Extraction process of environment-friendly blue pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Acquisition of Streptomyces sp. YLA0 strain producing environment-friendly blue pigment

[0049] Take a 3-30cm soil sample from the peony rhizosphere cultivated in the peony area of ​​Heze, Shandong Province. Take 5g of the soil sample and put it into a triangular flask (glass beads) filled with 45mL of sterile water. solution, diluted 10 times, took 0.1mL and spread it evenly on the modified Gaoshi No. 1 synthetic agar medium plate with a sterile glass coating rod, and placed it upside down in a 28°C incubator for 5-7d. Colonies producing blue water-soluble pigments were separated and purified by streaking three times in a row ( figure 1 and figure 2 ), the starting strain producing blue pigment, namely, Streptomyces sp. YLA11 strain can be obtained.

[0050] Inoculate the starting strain into the seed medium, collect and suspend the conidia of the starting strain with physiological saline, and make the spore concentration 1×10 8 - 10 9 cfu / ml spore suspen...

Embodiment 2

[0054] Example 2 Fermentation of Streptomyces sp. YLA0 strain producing environmentally friendly blue pigment to produce blue pigment

[0055] The method for producing blue pigment by adopting the Streptomyces sp. YLA0 strain producing environment-friendly blue pigment, placing the strain in a fermentation medium, and fermenting at 35-37°C for 2-9 days to obtain a fermentation broth The fermentation medium is made up of corn 20.0g, potassium nitrate 1.0g, sodium acetate 0.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, manganese sulfate 0.01g, ferrous sulfate 0.01g and water 1000mL. The pH of the fermentation medium is 7.2-7.4;

[0056] Manganese sulfate is added to the fermentation medium to stimulate the production of more blue pigment.

Embodiment 3

[0057] Example 3 Analysis of biological characteristics of Streptomyces sp. YLA0 strain producing environmentally friendly blue pigment

[0058] Gao's No. 1 medium: 20.0 g soluble starch, 0.5 g casein, potassium nitrate (KNO 3 ) 1.0g, sodium chloride (NaCl) 0.5 g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.5g, magnesium sulfate (MgSO 4 .7H 2 O) 0.5g, zinc sulfate (ZnSO 4 ) 0.01g, ferrous sulfate (FeSO 4 .7H 2 O) 0.01g, distilled water 1000ml, pH7.2-7.4.

[0059] 1. Morphological characteristics

[0060] On Gao's No. 1 medium, fluffy colonies can be formed when streaked and separated, and sparse light white fluffy colonies can be seen under the microscope in the early stage. Such as image 3 and Figure 4 The 48h colonies shown. Then gradually become thicker, white, but no blue pigment appears. Figure 5 (cultured for 60h), Figure 6 (cultured for 72h), Figure 7 (Cultivate for 96 hours), after 96 hours of cultivation, you can see the beginning of blue formatio...

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Abstract

The invention discloses an environment-friendly blue pigment extracting technology and belongs to the technical field of microorganism products. The acid precipitation, alcohol precipitation and ultrafiltration are combined, and the extracting technology is suitable for blue pigment separation. The technology is environmentally friendly, easy and convenient to implement and effective. Ethyl alcohol consumption can be greatly lowered, ethyl alcohol recycle is convenient, a part of protein in the pigment can be removed, the pigment quality is improved, pigment preservation is facilitated, and influence on the environment is small.

Description

technical field [0001] The invention relates to the field of microbial products, in particular to an extraction process of an environment-friendly blue pigment. Background technique [0002] With the development of cosmetics and food industries, people's demand for food coloring is increasing, and their safety requirements are also increasing. [0003] There are two main types of pigments used as food additives: one is artificial chemically synthesized pigments, the other is biological pigments; chemically synthesized pigments are mostly toxic and pose a greater threat to the human body, so the chemical pigments that are now allowed to be used are declining year by year ; Biochrome has been gradually favored by people because of its high safety and wide application range. [0004] Biological pigments can be divided into three categories according to their sources: plant-extracted pigments, animal-extracted pigments, and microbial pigments. Generally, when microbial ferment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00
Inventor 王宜磊孟国庆王文爽
Owner HEZE UNIV
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