Breeding method of high-photosynthetic-efficiency core collections of rape
A core germplasm and high-efficiency technology, applied in the fields of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of slow progress, difficult wide application, and high difficulty of high-light-efficiency breeding, and achieve favorable and sustainable development , avoid the effect of inaccurate results and accurate results
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Embodiment 1
[0041] Example 1: Breeding process of rapeseed high light efficiency core germplasm "7792-95 / 772 / / 772"
[0042] 1. 2006: Leaf chlorophyll and net photosynthetic rate were measured on the number 06GH118 (quoted from Dawangnan 17 male parent (04Sn254) genotype 7792-95 / 772 / / 772 in 2005). The measurement method is: continuously measure 14 individual plants, each individual plant measures the short-stalked leaf with the inverted 2 leaf position, and adopts the method of material rotation to reduce the difference in results caused by different measurement times; each material measures 2 pieces each time For the leaves, measure the materials used once within 30 minutes, a total of 7 times. The 6400-02BLED red and blue light source leaf chamber was used for the measurement, and the temperature in the leaf chamber was controlled at 25°C (TBlock), CO 2 The concentration is 400μmol / mL, and the light is 1100μmolm -2 ·s -1 , match at least 2 times every 30 minutes, and take the average ...
Embodiment 2
[0050] Example 2: Application of high light efficiency core germplasm "7792-95 / 772 / / 772"
[0051] 1. Transplantation of high-efficiency CMS CMS lines: Since 2012, it has been tested and found to be able to maintain, that is, the transfer of CMS lines has begun. Currently, 3 lines have been bred to the 4th generation;
[0052] 2. Application of chemical hybridization technology to prepare (CIMS) chemically induced hybrid combinations: 45 hybrid combinations were prepared in Zhangye Spring Sowing Base in Gansu Province in 2012, and in Dali Net Room in 2013 and 2014, and participated in the preliminary test.
[0053]3. The effect of directional selection on rapeseed high light efficiency core germplasm "7792-95 / 772 / / 772", see Table 1, Table 2 and figure 2 .
[0054] Table 1 - Breeding effect of main traits of "7792-95 / 772 / / 772" (comparison between 2014 and 2008)
[0055]
[0056]
[0057] Table 2- Effects of annual directional selection on chlorophyll content and net pho...
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