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C22orf26 gene and application of expression product of C22orf26 gene to preparing Parkinson diagnosis and treatment preparation

A C22ORF26, 1.C22ORF26 technology, applied in gene therapy, biological testing, biochemical equipment and methods, etc., can solve problems that cannot meet clinical needs, and achieve good application prospects, good stability, and fast response effects

Active Publication Date: 2016-01-13
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, genes related to hereditary PD have been found to include SNCA, LRRK2, DJ-1, PINK-1, Parkin, etc. These pathogenic genes need further research and are far from meeting clinical needs

Method used

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  • C22orf26 gene and application of expression product of C22orf26 gene to preparing Parkinson diagnosis and treatment preparation
  • C22orf26 gene and application of expression product of C22orf26 gene to preparing Parkinson diagnosis and treatment preparation
  • C22orf26 gene and application of expression product of C22orf26 gene to preparing Parkinson diagnosis and treatment preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 High-throughput sequencing and analysis

[0031] The peripheral blood samples of 15 Parkinson's patients and 9 healthy people were collected for RNA extraction. After RNA extraction, agarose gel electrophoresis was performed. From the electrophoresis results, it can be preliminarily judged whether the quality of the extracted RNA samples is qualified or not, and whether they can be used for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0032] The sequencing platform is the HiSeq2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) software Overall assessment of the quality of sequencing data, including base quality value distribution, quality value position dis...

Embodiment 2

[0033] Example 2 C22ORF26 Gene Expression in Peripheral Blood of Parkinson's Patients and Peripheral Blood of Healthy People

[0034] 1. Materials and methods

[0035] 1. Materials

[0036] The peripheral blood of 135 cases of Parkinson's patients and 33 cases of healthy people were collected, grouped and numbered.

[0037] 2. Method

[0038] 2.1 Extraction of total RNA from peripheral blood of Parkinson's and healthy people

[0039] use Reagent was used to extract RNA from samples, and the experimental operation was performed according to the product instructions.

[0040] Judgment criteria for RNA quality: the OD260 / OD280 value of the RNA sample is between 1.7 and 2.2; the electrophoretic pattern of total RNA has clear 28S and 18S bands; difference.

[0041] 2.2 Synthesis of cDNA by reverse transcription

[0042] use IIIReverseTranscriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out accor...

Embodiment 3

[0062] The cultivation of embodiment 3 cell line SN4741

[0063] 1. Materials

[0064] SN4741 cells were donated by the Fourth Military Medical University.

[0065] 2. Experimental method

[0066] cell culture

[0067] First, prepare a special medium for SN cells: add FBS54ml, D-glucose 16.2ml, L-glutamine 3.8ml and penicillin / streptomycin double antibody to 500ml DMEM (4.5g / LD-glucose). Then take out the frozen SN4741 cells from liquid nitrogen, put them in a 37°C water bath and thaw quickly within 1 min, suck out the cell suspension, transfer it into a centrifuge tube with the prepared medium, mix gently and pipette, 1200rpm Centrifuge for 5 minutes, remove the supernatant, continue to blow gently and evenly to form a cell suspension, then transfer to a culture dish, at 33 ° C, 5% CO 2 Cultured in a constant temperature incubator with 100% humidity. When the cells are attached to the wall and the density reaches 75-85%, they can be subcultured. First remove the original...

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Abstract

The invention relates to the field of biological medicine, in particular to a C22orf26 gene and an application of an expression product of the C22orf26 gene to preparing a Parkinson diagnosis and treatment preparation. An inventor carries out analysis with a bioinformatics method based on high-throughput sequencing results to carry out gene screening, and the candidate gene C22orf26 is selected. Further, it is proved that the C22orf26 gene and Parkinson have well correlation through a molecular biology experiment, and the C22orf26 gene can be used for preparing the auxiliary Parkinson diagnosis and treatment preparation, and has important clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of C22orf26 gene and its expression product in the preparation of reagents for Parkinson's diagnosis and treatment. Background technique [0002] Parkinson's disease (Parkinson's disease, PD) is a chronic progressive disease, currently incurable, it was first described by the British Parkinson in 1817, with the progress of PD research, we have a deeper understanding of this disease , it is clinically manifested as motor symptoms such as resting tremor, bradykinesia, muscle rigidity, and posture and gait disturbance and non-motor symptoms such as constipation, sleep disturbance, and abnormal mental behavior. At present, the pathogenesis of PD is still unclear, and it may be related to genetic factors, mitochondrial dysfunction, oxidative stress, immune abnormalities, cell apoptosis, and environmental factors. In PD, about 10% of patients are familial PD patients with ob...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61P25/16C12Q1/68G01N33/68
Inventor 杨承刚
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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