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Microbial oil preparation method

A technology of microbial oil and oil-producing microorganisms, applied in the direction of fat production, fat oil/fat production, fermentation, etc., to achieve the effect of reducing production costs, reducing preparation processes, and high quality crude oil

Inactive Publication Date: 2016-01-06
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing enzymatic degumming process is carried out after the crude oil is obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] With dinoflagellation as the fermentation strain, follow the following steps successively:

[0015] (1) fermentation to obtain a microbial fermentation broth rich in docosahexaenoic acid;

[0016] (2) After the above-mentioned fermented liquid is processed by a centrifuge, the solid content obtained is 23.1% concentrated fermented liquid;

[0017] (3) Take 1000g of the concentrated fermentation broth and put it into a 1L glass reaction bottle, adjust the pH value to 8.1, add 1.01g of alkaline protease, control the temperature at 45°C, cut for 2h, then add 1.0g of phospholipase A, and stir further React for 3 hours to obtain the enzymolysis solution.

[0018] (4) Boil the above-mentioned enzymolysis solution, then centrifuge with a high-speed centrifuge at 10000 rpm, separate the upper oil phase and carry out vacuum dehydration to obtain 81.1 g of microbial oil rich in docosahexaenoic acid.

[0019] (5) After testing, the phosphorus content in the microbial oil is 4.1p...

Embodiment 2

[0021] Using Microella as the fermentation strain, follow the steps below:

[0022] (1) fermentation to obtain a microbial fermentation broth rich in eicosapentaenoic acid;

[0023] (2) After the above-mentioned fermented liquid is separated by membrane, the concentrated fermented liquid obtained with a solids content of 18.5%;

[0024] (3) Take 5kg of the concentrated fermentation broth and put it into a 25L glass reactor, adjust the pH value to 7.01, add 5.1g alkaline protease, 5.1g helicase, control the temperature at 51°C, 25g phospholipase A, stir and shear React for 2 hours to obtain the enzymolysis solution.

[0025] (4) 10 L of n-hexane and 5 L of ethanol were added to the enzymolysis solution for extraction for 1 hour, and the extract was separated by sedimentation to obtain an extract, which was subjected to vacuum precipitation to obtain 377.3 g of microbial oil rich in eicosapentaenoic acid.

[0026] (5) After testing, the phosphorus content in the microbial oil ...

Embodiment 3

[0028] Using yeast as the fermentation strain, follow the steps below:

[0029] (1) Fermentation obtains a microbial fermentation broth rich in linolenic acid;

[0030] (2) After centrifuging the above-mentioned fermented liquid, the concentrated fermented liquid obtained with a solids content of 28.3%;

[0031] (3) Take 51kg of the concentrated fermentation broth and put it into a 300L reaction kettle, adjust the pH value to 5, control the temperature at 48°C, add 100.1g of helicase and 127.5g of phospholipase A, stir and cut for 5 hours to obtain an enzymolysis solution.

[0032] (4) Add 100 L of n-hexane and 50 L of ethanol to the above enzymolysis solution to extract for 1.5 h, settle and separate to obtain the extract, and vacuum precipitate the extract to obtain 4171.3 g of microbial oil rich in linolenic acid.

[0033] (5) After testing, the phosphorus content in the microbial oil is 2.9ppm.

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Abstract

The invention provides a microbial oil preparation method. The microbial oil preparation method comprises the steps that fermentation is conducted to obtain oil-producing microbial fermentation liquor; the fermentation liquor is concentrated to obtain concentrated fermentation liquor with the solid content higher than 10%; the PH value of the fermentation liquor is regulated between 5 and 8.1, phospholipase A is added into the fermentation liquor, the temperature is controlled between 38 DEG C and 51 DEG C, and the fermentation liquor is stirred and sheared for 2-6 hours; the oil phase and the water phase are separated to obtain microbial oil with the phosphorus content lower than 5 ppm. The microbial oil preparation method has the advantages that the degumming process and the microorganism wall breaking process are combined, so that oil preparation processes are reduced, and production cost is lowered; furthermore, the degumming process is conducted before the crude oil preparation process, so that produced crude oil is higher in quality.

Description

technical field [0001] The invention relates to a method for preparing microbial oil. Background technique [0002] At present, microbial oil is obtained by fermenting microbial strains to obtain a large number of microorganisms, and then performing pretreatment, extracting with organic solvents, and desolventizing to obtain microbial crude oil. The microbial crude oil already contains specific microbial oil components, so it can be sold as a product on the market. However, the microbial crude oil has many impurities and is not good in sense. Therefore, it is often necessary to further refine it to obtain microbial oil with better quality. Oil refining is usually divided into degumming, alkali refining, decolorization, deodorization and other processes. Among them, degumming is the basis of the entire oil refining process, and its main function is to remove phospholipids (hydratable phospholipids and non-hydratable phospholipids) in oil. Traditional degumming methods are d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B1/00
Inventor 李翔宇陆姝欢汪志明田勇周强易德伟
Owner CABIO BIOTECH WUHAN CO LTD
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