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Compound microbial preparation used for water body and sediment remediation and preparation method thereof

A technology of compound microorganisms and preparations, applied in the field of environmental remediation, can solve the problems of lack of efficient bacteria and mature technology, inability to act simultaneously with sediment and water bodies, and limited application scope, so as to improve the micro-ecosystem and restore biodiversity. The effect of improving self-purification ability

Inactive Publication Date: 2016-01-06
GUANGDONG PROVINCIAL ACADEMY OF ENVIRONMENTAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the concentration of pollutants in natural water bodies is relatively low. How to adapt bacteria to the new environment and how to increase the activity and number of microorganisms is the key to whether this technology can be widely used.
my country started late in the field of using microbial technology to remediate water pollution, and there is still a gap in technology compared with developed countries
The proportion of microbial remediation in pollution control is relatively small, mainly due to the lack of efficient strains and mature technologies
[0010] The scope of application of the existing technology is limited, generally only suitable for urban river water restoration, and most of them cannot act on the sediment and water body at the same time

Method used

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  • Compound microbial preparation used for water body and sediment remediation and preparation method thereof
  • Compound microbial preparation used for water body and sediment remediation and preparation method thereof
  • Compound microbial preparation used for water body and sediment remediation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Rural Pond

[0025] Chaodongtang in a village in Foshan, Guangdong covers an area of ​​3 mu, with a total water volume of 3000m 3 , the average daily discharge of domestic sewage is about 100-200m 3 , the villagers' domestic garbage is all over the pond, the water quality of the lake is poor, the smell is overflowing, and the degree of eutrophication is serious. In severe cases, the whole lake is covered with green algae, and the water quality near each sewage inlet is full of red worms.

[0026] The strains are mixed according to the following mass ratio to prepare a composite microbial preparation, Pediococcus lactis: Pediococcus pentosacea: Bacillus amyloliquefaciens: Pichia pastoris: Mycobacillus salidenitride=9:9:1:1:3, and then pass Vacuum-dried and stored in powder form.

[0027] The concentration of the Pediococcus lactis after expanding culture is not less than 5×10 7 CFU / mL, the concentration of the expanded culture of Pediococcus pentosaceus is ...

Embodiment 2

[0029] Example 2: Industrial Pollution Pond

[0030] A pond in Guangdong covers an area of ​​5,500 square meters (8 acres). It has been polluted by soy sauce brewing wastewater for a long time. The average water depth is 3 meters (excluding the bottom sludge layer). There is a lot of floating sludge on the surface, and the organic sludge layer at the bottom is about 1 meter. .

[0031] The strains are mixed according to the following mass ratio to prepare a composite microbial preparation, Pediococcus lactis: Pediococcus pentosaceae: Bacillus amyloliquefaciens: Pichia pastoris: Mycobacillus salidenitritis=9:9:7:5:1, and then pass Vacuum-dried and stored in powder form.

[0032] The concentration of the expanded culture of Pediococcus lactis is not less than 5×107 CFU / mL, the concentration of the expanded culture of Pediococcus pentosaceus is not less than 1×107 CFU / mL, and the concentration of the expanded culture of Bacillus amyloliquefaciens is not less than 5×107CFU / mL, t...

Embodiment 3

[0034] Example 3: Open Water Body

[0035] A river in Jiangmen City is located in the urban area, with a total length of about 3km and an average water depth of 2m. Due to the lack of sewage interception measures and weak awareness of environmental protection, the river is polluted by surrounding industrial sewage and domestic sewage, and has long been used as an urban sewage river. At the same time, a large amount of pollutants are deposited in the bottom of the river, forming a thick sludge layer. The water quality is inferior to Grade V, the water pollution is serious, the water is dark green or black, and has a stench.

[0036] The strains are mixed according to the following mass ratio to prepare a composite microbial preparation, Pediococcus lactis: Pediococcus pentosaceae: Bacillus amyloliquefaciens: Pichia pastoris: Mycobacillus salidenitritis=9:9:7:7:7, and then pass Vacuum-dried and stored in powder form.

[0037] The concentration of the Pediococcus lactis after ...

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Abstract

The invention discloses a compound microbial preparation used for water body and sediment remediation and a preparation method thereof. The preparation method comprises the following steps: mixing pediococcus acidilactici, pediococcus pentosaceus, bacillus amyloliquefaciens, pichia pastoris and virgibacillus halodenitrificans in a mass ratio of 9 to 9 to (1-7) to (1-7) to (1-7) to prepare a compound biological preparation and then carrying out vacuum drying to prepare powder, thus obtaining the compound microbial preparation. The compound microbial preparation used for water body and sediment remediation can be used for closed water bodies and open water bodies, can achieve the effects of rapidly reducing sediment, improving the self-cleaning capacities of the water bodies and improving the water quality, can not cause secondary pollution, is suitable for pollution control and remediation of river water in cities and remediation of industrially polluted closed water bodies and can achieve the effects of improving microecosystems of the water bodies and restoring the biodiversity of the water bodies.

Description

Technical field: [0001] The invention relates to the technical field of environmental remediation, in particular to a composite microbial preparation for water body and sediment restoration and a preparation method thereof. The composite microbial preparation can be used for closed water bodies and open water bodies, and can purify both bottom mud and water bodies effect. Background technique: [0002] Biological agents are high-efficiency bioactive bacterial agents made by advanced biotechnology and special production processes. The composition of biological agents can be summarized as microorganisms, enzymes and some substances that maintain microbial activity. [0003] Microbial agents are a very active research direction in the field of bioaugmentation technology. It has been widely used in various fields such as agriculture, industry, medicine, and food, and then applied to the field of environmental protection. [0004] Biological agents have unique advantages for tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C02F11/02C12R1/84C12R1/07C12R1/01
Inventor 黄志华叶向东汪永红李朝晖杨佘维王开演黄振雄
Owner GUANGDONG PROVINCIAL ACADEMY OF ENVIRONMENTAL SCI
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