Preparation method of antioxidant stock solution based on fermentation of sea-buckthorn and kudzu root and product thereof
An anti-oxidant and anti-oxidation technology, which is applied in the field of food processing, achieves remarkable effects and inhibits chain reactions
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Embodiment 1
[0036] The preparation of embodiment 1 antioxidant stoste
[0037] 1. Strain preparation
[0038] Saccharomyces cerevisiae (Saccharomycescerevisiae, ACCC20065) and Candidautilis (Candidautilis, ACCC20059) purchased from the China Agricultural Microorganism Culture Collection and Management Center were respectively transferred into Erlenmeyer flasks of potato liquid medium, at 28 ° C, 180- Shake culture at 220rpm for 48 hours, take out the medium after it becomes turbid, and store it in a 4°C refrigerator for later use; transfer Lactobacillus acidophilus (L.acidophilus, ACCC10637) and Bifidobacterium bifidum (ACCC11054) into MRS liquid culture respectively culture medium at 37°C for 48 hours, take it out after the medium is cloudy, and store it in a refrigerator at 4°C until use.
[0039] 2. Compound culture of bacteria:
[0040] (1) The strains cultivated in step 1 are weighed according to the following weight percentages: 20% of Saccharomyces cerevisiae, 20% of Candida util...
Embodiment 2
[0049] The preparation of embodiment 2 antioxidant liquid beverages
[0050] 1. Strain preparation: with embodiment 1;
[0051] 2. Compound culture of bacteria:
[0052] (1) The strains cultivated in step 1 are weighed by the following weight percentages: 25% of Saccharomyces cerevisiae, 25% of Candida utilis, 25% of Lactobacillus acidophilus, and 25% of Bifidobacterium;
[0053] (2) Composite culture: Put the strains weighed above into the raw materials with the ratio of 1:100: brown sugar 15%, hydrogen phosphate 1.5%, pure water 83.5%, put them into the fermenter and stir them evenly for 30 Cultivate in a semi-closed manner at ℃ for 1 day, then culture in an airtight manner, and cultivate for 10 days after the pH is less than 3.6.
[0054] 3. Solid-liquid fermentation:
[0055] (1) Crushing of raw materials: the dried seabuckthorn fruit was washed and dried, and crushed into 40 meshes with a grinder; the dried kudzu root fruit was crushed into 40 meshes with a grinder.
...
Embodiment 3
[0063] The preparation of embodiment 3 antioxidant solid drinks
[0064] 1. Strain preparation: with embodiment 1;
[0065] 2. Bacterial classification composite culture: with embodiment 2;
[0066] 3. Solid-liquid fermentation: with embodiment 2;
[0067] 4. Preparation of antioxidant stock solution: same as Example 2;
[0068] 5. Preparation of Antioxidant Solid Beverage:
[0069] The antioxidant stock solution was pre-cooled at -45°C in a freeze dryer, and vacuum freeze-dried into powder through 13-stage temperature programming to obtain antioxidant fermentation powder. Proportioning: 25% of the antioxidant fermented powder, erythritol 25%, lactose 40%, protective agent 2%, the antioxidant fermented powder, erythritol and lactose are stirred together in a granulator Granules, sprayed with a protective agent dissolved in an appropriate amount of water, the air inlet temperature of the granulator is 65°C, the outlet air temperature is 45°C, and the material is discharged ...
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