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A method for separating soluble boron in plants

A separation method and soluble technology, applied in the field of separation of soluble boron in plants, to achieve the effect of simple operation steps and strong operability

Inactive Publication Date: 2018-02-16
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between boron content, existing form, plant body fluid pH value, and boron isotope composition. The problem that needs to be solved urgently is the separation of soluble boron in plants, and then study its existing form and body fluid pH value, boron isotope composition. Correlation Between Changes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0023] (1) The plant organ samples were repeatedly washed with tap water and deionized water 5 times each, dried in a drying oven at 40°C, crushed with a plant grinder, and passed through a 60-mesh analytical sieve.

[0024] (2) Discard the piston part of the medical 2.5mL disposable syringe, remove the needle sheath, pull out and discard the needle stem, heat the thin end of the needle plug, flatten it while it is hot, and use it as a syringe stopper Put a solid-phase extraction sieve plate (Restek brand, product number 26017) with a diameter of 9.0 mm and a pore size of 20 μm in the syringe barrel, so that the sieve plate is placed in the syringe barrel close to the nipple, and the plane of the sieve plate is perpendicular to the syringe barrel ; Accurately weigh 0.2g of the plant sample, place it in the syringe barrel with a sieve plate and a plug, add 2 mL of deionized water into the barrel, and after the water and the plant sample are completely infiltrated, place the syri...

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Abstract

The invention discloses a method for separating soluble boron in plants. The steps are as follows: (1) washing, drying, crushing and sieving the plant samples; (2) weighing a certain amount of powder samples and placing them in a In the plugged syringe, add water to immerse the sample and oscillate ultrasonically; (3) Remove the plug of the syringe syringe, place the syringe syringe in a screw cap centrifuge tube, centrifuge and collect the filtrate; (4) Repeat the steps (2) Add water and shake and step (3), separate 5-10 times in total, and combine all filtrates, that is, completely separate the soluble boron in the plant sample. The present invention establishes a method for separating soluble boron in plants. The method can separate the soluble boron in the body fluid of plant samples from the insoluble boron in tissue residues. The method is simple, reliable and has wide application prospects , to provide new technical support for research in the fields of plant boron nutrition, boron physiology and biochemistry, boron biogeochemical cycle and other fields.

Description

technical field [0001] The invention relates to a method capable of completely separating soluble boron in plants. Background technique [0002] Boron is one of the micronutrient elements necessary for plants, and plays a very important role in plant growth and development, the establishment and development of plant reproductive organs, and plant photosynthesis. The rapid development of analytical testing has led to the development of boron determination methods. The current methods of boron determination include: spectrophotometry (formimine H acid method, curcumin method and beryllium reagent, etc.), fluorescence photometry, atomic absorption spectrometry (AAS), plasma emission spectrometry (ICP-OES), plasma mass spectrometry (ICP-MS), etc. These methods have improved the measurement accuracy of boron in plants and made the measurement results more reliable. At present, in the analysis and testing of boron in plants, various techniques (acid dissolution method, dry ashing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 徐庆彩
Owner LINYI UNIVERSITY
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