Indoor rice variety Magnaporthe oryzae resistance identification method
A technology for resistance identification and rice blast, applied in the agricultural field, can solve problems such as loss of resistance of disease-resistant varieties, and achieve the effect of saving labor and time.
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[0017] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0018] Isolation of single spore and preparation of spore suspension and inoculation method
[0019] Spray inoculation, spot inoculation, and injection inoculation are the three most widely used inoculation methods for rice blast. Through years of experiments, our research team combined the above three methods for indoor identification of rice blast resistance.
[0020] Use the spore shake-off method to separate monospores of Magnaporthe grisea: first soak the panicle blast specimens in sterile water for 48 hours, and then move them to a petri dish for 48 hours of moisturizing culture to allow them to sporulate. Water-absorbing paper, one end of the absorbent paper is made into a paper pillow, so that both ends of the specimen are in contact with the absorbent paper, and the lesion is suspended (temperature 26°C, light:dark=12h:12h). Wh...
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