Enzymolysis preparation method of anti-coagulation peptide

An anticoagulant peptide and enzymolysis technology, which is applied in the field of preparing polypeptides with anticoagulant activity, can solve the problems of low inhibition rate of thrombin inhibitory peptides, and achieve the effects of low price, abundant raw materials and satisfying large-scale industrial production.

Active Publication Date: 2015-12-23
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to solve the problem that the inhibitory rate of thrombin inhibitory peptide prepared from plant protein is relatively low, and to provide an enzymatic preparation method of antithrombin peptide

Method used

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Examples

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specific Embodiment approach 1

[0027] Specific embodiment 1: The enzymatic hydrolysis preparation method of the anticoagulant peptide in this embodiment is implemented according to the following steps:

[0028] 1. Soybean protein isolate is dissolved in distilled water to prepare soybean protein solution;

[0029] 2. Heat the soybean protein solution obtained in step 1 to 60-90°C for 5-10 minutes, adjust the pH of the system after cooling to room temperature, then add protease to carry out hydrolysis reaction, and obtain an enzymatic hydrolysis solution after the enzyme-inactivating treatment;

[0030] 3. Centrifuge the enzymolysis solution, collect the supernatant, and then filter it with a filter membrane with a molecular weight cut-off of less than 500 to obtain the filtered enzymolysis solution;

[0031] 4. Homogenize the filtered enzymolysis solution at 60-65°C and 15-30MPa to obtain a homogenized enzymolysis solution;

[0032] 5. Sterilizing the homogenized enzymolysis solution to obtain a sterilized...

specific Embodiment approach 2

[0040] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of the soybean protein solution in step 1 is 4wt%-12wt%. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0041] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the alkaline regulator described in step two is NaOH solution, KOH solution, sodium bicarbonate solution or potassium bicarbonate solution. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides an enzymolysis preparation method of an anti-coagulation peptide, belonging to the technical field of deep processing of food-borne protein, and aiming to solve the problem that the inhibition rate of the thrombin inhibition peptide prepared from vegetable protein as a source is relatively low. The preparation method comprises the following steps: (1) dissolving isolated soybean protein in distilled water to obtain a soybean protein solution; (2) heating the soybean protein solution at 60-90 DEG C, regulating the pH value of the system according to different kinds of protease, adding the protease to carry out hydrolysis reaction, and carrying out enzyme deactivation treatment to obtain an enzymolysis solution, wherein the protease is pepsin, trypsin, alkaline protease or compound protease; (3) filtering the enzymolysis solution; (4) homogenizing; (5) sterilizing the homogenized enzymolysis solution; and (6) drying the sterilized enzymolysis solution to obtain a soybean anti-coagulation peptide. The maximum thrombin inhibition rate of the soybean anti-coagulation peptide prepared from the soybean protein as a raw material can reach 40%.

Description

technical field [0001] The invention belongs to the technical field of food-derived protein deep processing, and in particular relates to a process for preparing a polypeptide with anticoagulant activity by using soybean or soybean protein as a raw material. Background technique [0002] Due to the bad living habits of modern people such as overeating, smoking and drinking, this dynamic balance is broken, which triggers the coagulation system in the blood, causing the blood to coagulate in the blood vessels to form thrombus. Cardiovascular and cerebrovascular thrombotic diseases have become the number one killer of human beings. At present, cardiovascular diseases cause 1.8 million premature deaths in Europe every year, and the epidemic trend of cardiovascular diseases in my country is not optimistic. According to the survey, the prevalence of cardiovascular disease in my country is 1.78% for men and 1.10% for women, and the prevalence increases with age. [0003] The drugs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 杜明王聪涂茂林麦树财石璞洁
Owner HARBIN INST OF TECH
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