Zymolytic tuna tablet for beautifying and fighting against free radicals and preparation method of zymolytic tuna tablet

A technology of tuna and free radicals, which is applied in the direction of anti-toxic agents, pill delivery, and medical raw materials derived from fish feed, etc., can solve the problems of inapplicable antioxidants, achieve the inhibition of lipid peroxidation, less irrelevant ingredients, and the content of active ingredients high effect

Inactive Publication Date: 2015-12-23
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research on tuna blood antioxidants in my country is still in a blank stage, and there are few reports on the practical enzymatic hydrolysis process and separation and purification
However, the methods for preparing antioxidants from soft-shelled turtles, mussels, and mussels that have been reported so far are not suitable for preparing antioxidants from tuna blood.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The enzymolyzed tuna beauty anti-free radical tablet and the preparation method thereof comprise using tuna blood as a raw material, wherein: in parts by weight: 15-20 parts of tuna blood enzymatic hydrolyzate dry powder; 4-8 parts of starch; 5-5 parts of dextrin 12 parts; 0.1-0.4 parts of tartaric acid; 0.2-0.4 parts of magnesium stearate. The finished product is safe and non-toxic, has strong antioxidant activity and is easy to digest and absorb, and can eliminate free radicals and inhibit lipid peroxidation. The combined use of tartaric acid and magnesium stearate has unexpectedly achieved a good effect of maintaining antioxidant components. Anti-free radical tablets also contain ethanol. Ethanol is a good solvent in the preparation of anti-free radical tablets, and it is easy to separate and relatively safe. The preparation steps of tuna blood enzyme hydrolyzate are as follows:

[0019] 1) After the tuna blood is deodorized, adjust the pH with phosphate buffer, a...

Embodiment 2

[0033](1) Take 500mL of tuna blood, add 2000mL of No. 6 light gasoline to remove the fishy smell, remove the No. 6 light gasoline, and adjust the pH to 7 with phosphoric acid buffer;

[0034] (2) Add 5g of trypsin to the above tuna dilution, enzymatically hydrolyze at 55°C for 3h, then rapidly raise the temperature to 100°C and maintain for 15min;

[0035] (3) Centrifuge the enzymatic solution at 8000r / min and 4°C for 10min, and take the supernatant;

[0036] (4) Prepare the obtained enzymatic hydrolyzate into a solution with a concentration of 20mg / mL, add it to a macroporous resin chromatography column for desalination, and then use 55% ethanol for analysis, and remove the ethanol by rotary evaporation under 40°C under low pressure, and the concentrated solution Freeze-drying is carried out to obtain dry powder of the desalted enzyme hydrolyzate.

[0037] (5) Take 20g of dry powder of enzymatic hydrolyzate, 8g of starch, 12g of dextrin, 0.4g of tartaric acid, appropriate am...

Embodiment 3

[0040] (1) Take 500mL of tuna blood, add 2000mL of No. 6 light gasoline to remove the fishy smell, remove the No. 6 light gasoline, and adjust the pH to 7 with phosphoric acid buffer;

[0041] (2) Add 5g of trypsin to the above tuna dilution, enzymatically hydrolyze at 55°C for 3h, then rapidly raise the temperature to 95°C and maintain for 10min;

[0042] (3) Centrifuge the enzymatic solution at 8000r / min and 4°C for 10min, and take the supernatant;

[0043] (4) Prepare the obtained enzymatic hydrolyzate into a solution with a concentration of 18mg / mL, add it to a macroporous resin chromatography column for desalination, and then use 45% ethanol for analysis, and remove the ethanol by rotary evaporation under 40°C under low pressure, and the concentrated solution Freeze-drying is carried out to obtain dry powder of the desalted enzyme hydrolyzate.

[0044] (5) Take 20g of dry powder of enzymatic hydrolyzate, 8g of starch, 12g of dextrin, 0.4g of tartaric acid, appropriate am...

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PUM

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Abstract

The invention discloses a zymolytic tuna tablet for beautifying and fighting against free radicals and a preparation method of the zymolytic tuna tablet. Tuna is adopted as a raw material, and the zymolytic tuna tablet is prepared from the following materials in parts by weight: 15 to 20 parts of tuna blood zymolyte dry powder, 4 to 8 parts of starch; 5 to 12 parts of dextrin, 0.1 to 0.4 parts of tartaric acid and 0.2 to 0.4 parts of magnesium stearate. A finished product provided by the invention is safe without toxic and side effects, strong in antioxidant activity and easy in digestion and absorption, meanwhile, can eliminate the free radicals and inhibit lipid peroxidation. An extraordinary effect of well keeping antioxidant components is achieved by the match use of the tartaric acid and the magnesium stearate. Therefore, the zymolytic tuna tablet disclosed by the invention has the advantages of fast extraction, high effective component content, safety, non-toxic side effects, fewer unrelated components, strong antioxidant activity and easy digestion and absorption, and being capable of eliminating the free radicals and inhibiting the lipid peroxidation.

Description

technical field [0001] The invention relates to an antioxidant product and a preparation method thereof, in particular to an enzymolyzed tuna cosmetic anti-free radical tablet and a preparation method thereof. Background technique [0002] Antioxidants are a class that can prevent the adverse effects of oxygen, help capture and neutralize free radicals, and eliminate free radical damage to the human body. The high-efficiency free-radical-removing tablet of the present invention is added with tuna blood, overcomes the shortage of trace elements in previous tablets, can efficiently remove free-radicals, and promotes cell regeneration at the same time. Moreover, the toxicity of antioxidants prepared from tuna blood is much lower than that of synthetic ones. It has the advantages of being safe and having no toxic and side effects. [0003] Tuna is one of the important operating fish species in the world's pelagic fisheries. According to the statistics of the Food and Agricultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/60A61K9/20A61K47/12A61K47/10A61P39/06A61P17/00
Inventor 胡世伟吴凯强宋文东郭健纪丽丽李世杰王亚宁姜维蔡璐
Owner ZHEJIANG OCEAN UNIV
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