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Preparation method of mannan oligosaccharide synbiotic enteric microcapsules

A technology of mannose oligosaccharide and synbiotics, which is applied in the field of health care products with probiotic effect, can solve the problems of easy death of live bacteria, high number of live bacteria, poor effect, etc., so as to adjust intestinal microecology, promote body health, Improve the effect of intestinal flora imbalance

Active Publication Date: 2015-12-23
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The live bacteria of probiotic products are easy to die during transportation and storage, resulting in poor effect
In addition, almost all products in the market are oral preparations, and the survival rate of live bacteria in the digestive tract is very low. When it reaches the hindgut for fermentation, it cannot guarantee a high number of live bacteria
[0006] At present, most of the synbiotics products on the market only use a simple combination of probiotics and prebiotics, and the products are all gastric-soluble, which cannot resist the harsh environment of the gastrointestinal tract, resulting in the survival of probiotics in the hindgut. The number of bacteria has dropped significantly, and the effect of synbiotics has dropped significantly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Various component ratios of synbiotics:

[0032] 20% sugar solution, 2% fungus slime, 10% skimmed milk powder, 5% gum arabic, 10% coating agent.

[0033] Synbiotics preparation process:

[0034] 1. Enzymatic hydrolysis: add konjac powder at a ratio of 5% to endo-type β-mannanase, and enzymatically hydrolyze at 55°C for 8 hours.

[0035] 2. Separation: the enzymatic hydrolyzate 1 was separated by centrifugation, and the precipitate was removed to obtain the enzymatic hydrolyzate 2.

[0036] 3. Membrane separation: use a filter membrane with a cut-off of 3000-10000 to perform membrane separation on the above enzymatic solution 2 to obtain a sugar solution with a molecular weight lower than 10000.

[0037] 4. Concentration: vacuum concentrate the above sugar solution 3 to 1 / 10 of the original volume.

[0038] 5. Ethanol precipitation: Add absolute ethanol to the above concentrated solution 4 to a final concentration of 60%-90%.

[0039] 6. Separation: The ethanol preci...

example 2

[0047] Various component ratios of synbiotics:

[0048] 10% sugar solution, 2% fungus slime, 5% skimmed milk powder, 0.5% gum arabic, 1% coating agent.

[0049] 1. Enzymatic hydrolysis: add konjac powder at a ratio of 5% to endo-type β-mannanase, and enzymatically hydrolyze at 55°C for 8 hours.

[0050] 2. Separation: the enzymatic hydrolyzate 1 was separated by centrifugation, and the precipitate was removed to obtain the enzymatic hydrolyzate 2.

[0051]3. Membrane separation: use a filter membrane with a cut-off of 3000-10000 to perform membrane separation on the above enzymatic solution 2 to obtain a sugar solution with a molecular weight lower than 10000.

[0052] 4. Concentration: vacuum concentrate the above sugar solution 3 to 1 / 10 of the original volume.

[0053] 5. Ethanol precipitation: Add absolute ethanol to the above concentrated solution 4 to a final concentration of 60%-90%.

[0054] 6. Separation: The ethanol precipitation of the above 5 was separated by ce...

example 3

[0062] Various component ratios of synbiotics:

[0063] 15% sugar solution, 3% fungus slime, 8% skimmed milk powder, 0.5% gum arabic, 5% coating agent.

[0064] 1. Enzymatic hydrolysis: add konjac powder at a ratio of 5% to endo-type β-mannanase, and enzymatically hydrolyze at 55°C for 8 hours.

[0065] 2. Separation: the enzymatic hydrolyzate 1 was separated by centrifugation, and the precipitate was removed to obtain the enzymatic hydrolyzate 2.

[0066] 3. Membrane separation: use a filter membrane with a cut-off of 3000-10000 to perform membrane separation on the above enzymatic solution 2 to obtain a sugar solution with a molecular weight lower than 10000.

[0067] 4. Concentration: vacuum concentrate the above sugar solution 3 to 1 / 10 of the original volume.

[0068] 5. Ethanol precipitation: Add absolute ethanol to the above concentrated solution 4 to a final concentration of 60%-90%.

[0069] 6. Separation: The ethanol precipitation of the above 5 was separated by c...

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Abstract

The invention relates to an intestine targeting synbiotic product. The intestine targeting synbiotic product contains oligomeric mannose and bacterium lacticum, wherein an oligomeric mannose composition is obtained through enzymolysis of konjak mannan, after refining, the content of monosaccharide is lower than 5%, the content of C2-C7 saccharides is higher than 90%, and the viable count of probiotics is not less than 2.5*10<11> CFU / g. In the preparation technology, through duplicate protection, the stability of the synbiotic is guaranteed, the benefitting effect of the synbiotic is improved, and the intestine targeting synbiotic product has the effects of improving intestinal flora disequilibrium and regulating the intestinal microecology, so that the intestine targeting synbiotic product is an environmentally-friendly microecological preparation.

Description

technical field [0001] The invention generally relates to a preparation method of mannan oligosaccharide synbiotic enteric-coated microcapsules, which belongs to health products with probiotic effect. Background technique [0002] In recent years, more and more attention has been paid to the intestinal microecology, which has been considered as the second organ of the human body and is closely related to human health and diseases. With the rapid development of the economy, the diet structure of human beings has undergone great changes, coupled with the high pressure of life and work, and irregular living habits, etc., it is easy to lead to the disorder of the micro-ecological system of the human body and the imbalance of intestinal flora, which in turn leads to a series of diseases. Chronic diseases such as irritable bowel syndrome, constipation, intestinal flora disturbance, etc. In addition, in recent years, journals such as Science and Nature have published articles one ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/214A23L1/28A23L1/00A23L1/09A23L1/05A23L1/29
CPCA23V2002/00A23V2200/3204A23V2250/618A23V2250/5022
Inventor 蒋敏史劲松许正宏马延和李恒罗春勤
Owner JIANGNAN UNIV
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