High-efficiency propagation method of Zinnia elegans virus-free seedling
A kind of technology of virus-free seedling and zinnia
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[0047] In this embodiment, the method for the efficient multiplication of detoxified zinnia seedlings, the experimental variety was selected as Fangfei No. 2, and the implementation time was 2014-2015. The city revitalizes the flower garden, and the tissue culture seedlings are transplanted in the flower test base of Dongxin Farm, Lianyungang City. The detailed steps are as follows:
[0048] 1) Pre-treatment of Fangfei No. 2 virus-free seedlings:
[0049] Rinse Fangfei No. 2 virus-free seedlings with tap water repeatedly first, wash once with a brush dipped in detergent, then rinse with ultra-pure water, soak in 70% alcohol for 15 seconds, and then rinse twice with sterile water, 0.1% Add a few drops of Tween 20 solution to the mercuric acid and soak for 5 minutes, rinse with sterile water 4-5 times, blot dry with filter paper, and transfer the petiole and receptacle of Fangfei No. 2 virus-free seedlings into adventitious bud induction medium (the formula is: MS+0.5mg / LKT+1.5...
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